A potato Sus3 sucrose synthase gene contains a context-dependent 3' element and a leader intron with both positive and negative tissue-specific effects

The Plant Cell
H FuW D Park

Abstract

To examine which sequences are involved in regulating the potato sucrose synthase gene Sus3-65, we examined a series of deletion and substitution constructs in transgenic potato and tobacco plants. In a construct containing 3.9 kb of 5' flanking region, substitution of the native 3' sequence with the nopaline synthase 3' sequence and deletion of the leader intron did not significantly affect expression in vegetative tissues. However, in a construct containing only 320 bp of 5' flanking region, these changes had marked effects. Replacing the native 3' sequences with nopaline synthase 3' sequences caused a six- to 20-fold increase in expression in vascular tissue, and removing the leader intron almost completely abolished expression in potato plants. Surprisingly, removal of the leader intron from either the full-length construct or a construct containing only 320 bp of 5' flanking sequence reduced expression in vascular tissue of tobacco anthers at later stages of development but increased expression in pollen by more than 100-fold.

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Citations

Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·E -G. NoC A. Loopstra
Aug 1, 2002·The Plant Journal : for Cell and Molecular Biology·Katrin CzempinskiBernd Mueller-Roeber
Sep 22, 2010·Journal of Experimental Botany·Laura MorelloDiego Breviario
Sep 3, 2011·Genome Génome / Conseil National De Recherches Canada·Kerri B McIntoshPeta C Bonham-Smith
Oct 24, 2013·Plant Molecular Biology·Mark KinkemaJames L Dale
Mar 9, 2013·Bioscience, Biotechnology, and Biochemistry·Muhammad Abul Kalam AzadShin Kore-eda
Aug 19, 2004·The Plant Cell·Shui WangXiao-Ya Chen
Sep 27, 2000·Critical Reviews in Biochemistry and Molecular Biology·H Winter, S C Huber
Jun 14, 2002·FEBS Letters·Luciana O FrancoDulce E de Oliveira
Jun 11, 2003·The Plant Journal : for Cell and Molecular Biology·Marcelo A GermanDavid Granot
Jul 6, 2013·Biotechnology Advances·Abdellatif BahajiJavier Pozueta-Romero
Dec 13, 2001·Journal of Experimental Botany·Akira KomatsuTomoya Akihama

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