PMID: 7506556Oct 1, 1993Paper

A potent, neutralizing human monoclonal antibody against a unique epitope overlapping the CD4-binding site of HIV-1 gp120 that is broadly conserved across North American and African virus isolates

AIDS Research and Human Retroviruses
Abraham PinterS A Tilley

Abstract

A human monoclonal antibody (HuMAb), 5145A, against HIV-1 gp120 was isolated from an asymptomatic, seropositive hemophiliac. The epitope of this HuMAb was destroyed by reduction of gp120 disulfide bonds, but not by removal of N-linked carbohydrates. This epitope overlaps the CD4-binding site of gp120, because binding of 5145A to gp120 is inhibited by soluble CD4 and by 1125H, a previously described HuMAb directed toward the CD4-binding site. However, the 5145A epitope differs from those of 1125H and other anti-CD4-binding site HuMAbs previously described, as documented by the viral strain specificity of 5145A and its reactivity with a panel of gp120 mutants. Specifically, 5145A reacted with 14 of 15 HIV-1 isolates tested, including 9 isolates from the Central African Republic, 6 of which were not recognized by 1125H. Partial epitope mapping of 5145A, using a series of gp120 mutants, demonstrated its lack of sensitivity to mutations in residues 257 and 427, contrasting with a marked sensitivity to mutations in residues 368 and 370. This pattern of reactivity distinguishes its epitope from that of any HuMAb against the CD4-binding site region described to date. In addition, 5145A exhibited potent and essentially equivalent neutra...Continue Reading

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Citations

Jun 14, 2003·Vaccine·Chongbin ZhuChin Ho Chen
Mar 25, 2004·Nature Reviews. Immunology·Susan Zolla-Pazner
Jan 1, 1995·AIDS Research and Human Retroviruses·S DemariaY Bushkin
May 1, 1995·Journal of Medical Primatology·K A Kent
Aug 18, 2005·European Journal of Immunology·Michael TuenCatarina E Hioe
Aug 3, 2004·Expert Review of Vaccines·Indresh K SrivastavaSusan W Barnett
May 19, 2012·Current Opinion in Immunology·Leonidas Stamatatos
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuxian HeAbraham Pinter

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