A Potential Biocontrol Agent Streptomycesviolaceusniger AC12AB for Managing Potato Common Scab

Frontiers in Microbiology
Arslan SarwarAndreas Bechthold

Abstract

Potato common scab (PCS) is an economically important disease worldwide. In this study we demonstrated the possible role of Streptomyces violaceusniger AC12AB in controlling PCS. Isolates of Streptomyces scabies were obtained from CS infected tubers collected from Maine United States, which were confirmed by morphological and molecular analysis including 16S rRNA sequencing and RFLP analysis of amplified 16S-23S ITS. Pathogenicity assays related genes including txtAB, nec1, and tomA were also identified in all S. scabies strains through PCR reaction. An antagonistic bacterial strain was isolated from soil in Punjab and identified as S. violaceusniger AC12AB based on 16S rRNA sequencing analysis. Methanolic extract of S. violaceusniger AC12AB contained azalomycin RS-22A which was confirmed by 1H and 13C-NMR, 1H/1H-COSY, HMBC and HMQC techniques. S. violaceusniger AC12AB exhibited plant growth promotion attributes including Indole-3-acetic acid production with 17 μgmL-1 titers, siderophores production, nitrogen fixation and phosphates solubilization potential. When tubers were inoculated with S. violaceusniger AC12AB, significant (P < 0.05) PCS disease reduction up to 90% was observed in greenhouse and field trials, respectively....Continue Reading

References

Jan 1, 1987·Analytical Biochemistry·B Schwyn, J B Neilands
Aug 1, 1971·Canadian Journal of Microbiology·W A Rice, E A Paul
May 7, 2004·Nature·Johan A KersRosemary Loria
Feb 3, 2005·Molecular Microbiology·Johan A KersRosemary Loria
Jun 18, 2005·Journal of Applied Microbiology·J S HanJ W Suh
Feb 1, 1991·Applied and Environmental Microbiology·J M BricS E Silverstone
Jan 1, 1951·Plant Physiology·S A Gordon, R P Weber
May 25, 2006·Annual Review of Phytopathology·Rosemary LoriaMadhumita Joshi
Sep 12, 2007·Journal of Natural Products·Kathrin SchneiderRoderich D Süssmuth
Oct 9, 2007·Molecular Microbiology·Madhumita V JoshiRosemary Loria
Apr 9, 2008·Antonie van Leeuwenhoek·Rosemary LoriaDonna M Gibson
Sep 9, 2008·Bioscience, Biotechnology, and Biochemistry·Masahiro TagawaYoichi Kamagata
Nov 6, 2010·Current Protocols in Microbiology·Madan K KharelJürgen Rohr
Sep 4, 2012·Nature Chemical Biology·Sarah M BarryGregory L Challis
Nov 26, 2014·Molecular Plant-microbe Interactions : MPMI·Joanna K FyansDawn R D Bignell
Aug 19, 2015·Frontiers in Microbiology·Soumitra Paul ChowdhuryRainer Borriss
Sep 17, 2017·The Science of the Total Environment·Martina HvězdováJakub Hofman
Oct 16, 2018·Frontiers in Microbiology·Hefa Mangzira KemungLearn-Han Lee
Nov 1, 2003·Plant Disease·Duck Hwan ParkChun Keun Lim

❮ Previous
Next ❯

Datasets Mentioned

BETA
JX185642
JQ990311
MG725948.1
MH388022.1
KU560917.1

Methods Mentioned

BETA
PCR
electrophoresis
Assay
acetylene reduction
NMR

Software Mentioned

SPSS Statistics
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.