A potential central role of Thaumarchaeota in N-Cycling in a semi-arid environment, Fort Stanton Cave, Snowy River passage, New Mexico, USA

FEMS Microbiology Ecology
Jason C KimbleDiana E Northup

Abstract

Low biomass and productivity of arid-land caves with limited availability of nitrogen (N) raises the question of how microbes acquire and cycle this essential element. Caves are ideal environments for investigating microbial functional capabilities, as they lack phototrophic activity and have near constant temperatures and high relative humidity. From the walls of Fort Stanton Cave (FSC), multicolored secondary mineral deposits of soil-like material low in fixed N, known as ferromanganese deposits (FMD), were collected. We hypothesized that within FMD samples we would find the presence of microbial N cycling genes and taxonomy related to N cycling microorganisms. Community DNA were sequenced using Illumina shotgun metagenomics and 16S rRNA gene sequencing. Results suggest a diverse N cycle encompassing several energetic pathways including nitrification, dissimilatory nitrate reduction and denitrification. N cycling genes associated with assimilatory nitrate reduction were also identified. Functional gene sequences and taxonomic findings suggest several bacterial and archaeal phyla potentially play a role in nitrification pathways in FSC and FMD. Thaumarchaeota, a deep-branching archaeal division, likely play an essential and po...Continue Reading

References

Aug 1, 1990·Applied and Environmental Microbiology·S J GiovannoniN R Pace
Jun 28, 1996·Science·S M SarbuB K Kinkle
Oct 6, 1998·Applied and Environmental Microbiology·L Y Stein, D J Arp
Jan 27, 1999·Bioinformatics·S R Eddy
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Nov 28, 2001·Applied and Environmental Microbiology·K TakaiJ K Fredrickson
Nov 27, 2002·Astrobiology·P J BostonR T Schelble
Dec 3, 2003·Environmental Microbiology·Diana E NorthupClifford N Dahm
Apr 7, 2004·Science·J Craig VenterHamilton O Smith
Mar 5, 2005·Applied and Environmental Microbiology·Annette BollmannMette H Nicolaisen
May 5, 2005·Applied and Environmental Microbiology·Gerhard J HerndlJakob Pernthaler
Sep 24, 2005·Nature·Martin KönnekeDavid A Stahl
Nov 1, 1979·Applied and Environmental Microbiology·K BancroftM Alexander
Apr 20, 2006·Trends in Microbiology·Engràcia CostaJan-Ulrich Kreft
May 17, 2006·Biotechnology and Bioengineering·Regina Nogueira, Luís F Melo
Aug 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Cornelia WuchterJaap S Sinninghe Damsté
Aug 18, 2006·Nature·S LeiningerC Schleper
Aug 19, 2007·Applied and Environmental Microbiology·John R SpearNorman R Pace
Nov 10, 2007·FEMS Microbiology Ecology·Lydie HerfortJaap S Sinninghe Damsté
Jan 22, 2008·Environmental Microbiology·José R de la TorreDavid A Stahl
Feb 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Roland HatzenpichlerMichael Wagner
Feb 16, 2008·Nature Reviews. Microbiology·Céline Brochier-ArmanetPatrick Forterre
Aug 30, 2008·Applied and Environmental Microbiology·Gerhard W WeidlerHelga Stan-Lotter
Jun 6, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Paula P PerissinottiFacundo Luna
Oct 13, 2009·FEMS Microbiology Ecology·Lejla PasićBlagajana Herzog-Velikonja
Mar 10, 2010·BMC Bioinformatics·Doug HyattLoren J Hauser
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Apr 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·C B WalkerD A Stahl
Jul 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sebastian LückerHolger Daims
Aug 17, 2010·Bioinformatics·Robert C Edgar
Nov 19, 2010·The ISME Journal·Scott T BatesNoah Fierer
Apr 26, 2011·Methods in Enzymology·Willm Martens-Habbena, David A Stahl
May 7, 2011·Current Opinion in Microbiology·Michael PesterMichael Wagner
Jul 1, 2011·The ISME Journal·Daniel S JonesJennifer L Macalady

❮ Previous
Next ❯

Citations

Aug 26, 2020·Biological Reviews of the Cambridge Philosophical Society·Stefano MammolaPedro Cardoso
Feb 15, 2020·FEMS Microbiology Letters·Jeffrey J MarlowJens Kallmeyer
Jan 17, 2021·Applied and Environmental Microbiology·Hai-Zhen ZhuShuang-Jiang Liu
Mar 2, 2021·PeerJ·Anastasiia K KimeklisEvgeny V Abakumov
Mar 23, 2021·Environmental Microbiology Reports·Yongchang ZhengWenjun Jiang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.