A Potential Compensatory Role of Panx3 in the VNO of a Panx1 Knock Out Mouse Model

Frontiers in Molecular Neuroscience
Paige Whyte-FagundesGeorg Zoidl

Abstract

Pannexins (Panx) are integral membrane proteins, with Panx1 being the best-characterized member of the protein family. Panx1 is implicated in sensory processing, and knockout (KO) animal models have become the primary tool to investigate the role(s) of Panx1 in sensory systems. Extending previous work from our group on primary olfaction, the expression patterns of Panxs in the vomeronasal organ (VNO), an auxiliary olfactory sense organ with a role in reproduction and social behavior, were compared. Using qRT-PCR and Immunohistochemistry (IHC), we confirmed the loss of Panx1, found similar Panx2 expression levels in both models, and a significant upregulation of Panx3 in mice with a global ablation of Panx1. Specifically, Panx3 showed upregulated expression in nerve fibers of the non-sensory epithelial layer in juvenile and adult KO mice and in the sensory layer of adults, which overlaps with Panx1 expression areas in WT populations. Since both social behavior and evoked ATP release in the VNO was not compromised in KO animals, we hypothesized that Panx3 could compensate for the loss of Panx1. This led us to compare Panx1 and Panx3 channels in vitro, demonstrating similar dye uptake and ATP release properties. Outcomes of this s...Continue Reading

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Citations

Mar 29, 2019·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·J M AbitbolS Penuela
Mar 13, 2020·Journal of Neurochemistry·Albert K YeungMichael F Jackson
Jun 14, 2020·Scientific Reports·Nickie SafarianGeorg Zoidl
Jul 13, 2021·Purinergic Signalling·Michael KovalBrant E Isakson
Jul 13, 2021·Purinergic Signalling·Brooke L O'Donnell, Silvia Penuela

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