A potential plasmid-curing agent, 8-epidiosbulbin E acetate, from Dioscorea bulbifera L. against multidrug-resistant bacteria

International Journal of Antimicrobial Agents
Varsha ShriramM G Shitole

Abstract

Bioassay-guided fractionation of an aqueous methanolic extract of Dioscorea bulbifera L. bulbs was performed using organic solvents. A novel plasmid-curing compound was identified as 8-epidiosbulbin E acetate (EEA) (norditerpene) on the basis of modern spectroscopic analysis and X-ray crystallography. EEA exhibited broad-spectrum plasmid-curing activity against multidrug-resistant (MDR) bacteria, including vancomycin-resistant enterococci. EEA cured antibiotic resistance plasmids (R-plasmids) from clinical isolates of Enterococcus faecalis, Escherichia coli, Shigella sonnei and Pseudomonas aeruginosa with 12-48% curing efficiency. The reference plasmids of Bacillus subtilis (pUB110), E. coli (RP4), P. aeruginosa (RIP64) and Salmonella typhi (R136) were cured with efficiency ranging from 16% to 64%. EEA-mediated R-plasmid curing decreased the minimal inhibitory concentration of antibiotics against MDR bacteria, thus making antibiotic treatment more effective. The antibiotic resistance pattern revealed that the compound was effective in the reversal of bacterial resistance to various antibiotics. In addition, the compound did not show any cytotoxicity against a broad range of human cancer cell lines, namely MCF-7 (breast cancer),...Continue Reading

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Citations

Nov 28, 2012·BMC Complementary and Alternative Medicine·Victor KueteNamrita Lall
Apr 18, 2014·Chemical & Pharmaceutical Bulletin·Ying TangYong-hui Zhang
Jul 26, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Sougata GhoshBalu A Chopade
Jan 8, 2011·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Min MaLuyong Zhang
May 11, 2010·Phytochemistry·Hai LiuZheng-Tao Wang
Apr 14, 2010·Phytochemistry·Lolona RakotobeBernard Bodo
Aug 20, 2015·Chemical Research in Toxicology·Dongju LinJiang Zheng
Jul 30, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Pei-Fen SuNing-Sun Yang
Jul 20, 2016·Natural Product Reports·Rongtao LiKuo-Hsiung Lee
Aug 8, 2018·FEMS Microbiology Reviews·Michelle M C BucknerLaura J V Piddock
Sep 4, 2014·Asian Pacific Journal of Tropical Biomedicine·Mubo A Sonibare, Adedapo A Adeniran
Nov 6, 2008·Animal Health Research Reviews·Douglas R CallAshish A Sawant
Aug 10, 2021·Frontiers in Pharmacology·Tushar KhareVinay Kumar

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