A potential role for adrenomedullin as a local regulator of bone growth

Endocrinology
D NaotJ Cornish

Abstract

Bone remodeling is a complex process of coordinated resorption and formation of bone, which is regulated by systemic hormones and by local factors. We have previously shown that the peptide hormone adrenomedullin is mitogenic to osteoblastic cells in vitro and that it promotes bone growth in vivo. The aim of the present study was to characterize the expression of molecules that may mediate adrenomedullin signaling in osteoblasts and to investigate the expression of adrenomedullin itself in these cells. The first adrenomedullin receptor that was cloned is the seven-transmembrane G protein-coupled receptor, L1. Two additional receptors for adrenomedullin, which arise from interactions between calcitonin receptor-like receptor and receptor activity modifying proteins 2 or 3, have now been described. In the current study, we used RT-PCR and Northern blot analysis to demonstrate that messenger RNA for the three adrenomedullin receptors, as well as for adrenomedullin itself, is expressed in primary rat osteoblasts. Treating primary osteoblasts with transforming growth factor-beta and insulin-like growth factor-I moderately reduced adrenomedullin RNA levels, whereas PTH had no effect. We have shown by immunocytochemistry that adrenome...Continue Reading

Citations

Jan 16, 2013·The Bulletin of Tokyo Dental College·Masakazu TazakiKenji Sueishi
Aug 3, 2010·Rheumatology International·Pooneh Salari SharifBagher Larijani
Jul 30, 2014·Frontiers in Immunology·Jorine J L P WillemsChristopher D Gregory
Jul 26, 2016·Frontiers in Physiology·Sonia Martínez-HerreroAlfredo Martínez
Feb 19, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Liesbeth van WesenbeeckWim Van Hul
Aug 10, 2011·Journal of Cellular Biochemistry·Sylvie Lausson, Michèle Cressent
Apr 13, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nobuhiro FukushimaMasayasu Kojima
Oct 18, 2007·Journal of Cellular Physiology·Benjamin UzanMichèle Cressent
Aug 1, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Susannah O'SullivanAndrew Grey
Dec 13, 2018·Physiological Reviews·Dorit NaotJillian Cornish
Aug 5, 2005·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·S Y S WongP C Leung
Mar 22, 2006·Expert Opinion on Therapeutic Targets·Mario A GarcíaAlfredo Martínez
Nov 13, 2009·Current Opinion in Endocrinology, Diabetes, and Obesity·Iris Pl WongHerbert Herzog
Jan 31, 2003·American Journal of Physiology. Endocrinology and Metabolism·I VillaF Guidobono
Apr 14, 2006·American Journal of Physiology. Cell Physiology·I VillaF Guidobono
Sep 24, 2020·International Journal of Endocrinology·Jingbo XieQingshan Sun
Feb 6, 2017·Journal of Peptide Science : an Official Publication of the European Peptide Society·Ria SchönauerAnnette G Beck-Sickinger
Oct 23, 2020·Journal of Biomedical Materials Research. Part a·Chunxiu MengFuzhai Cui
Mar 21, 2003·Biochemical and Biophysical Research Communications·Etsuo ChosaNaoya Tajima
Oct 7, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Toshio KukitaAkiko Kukita

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.