A potential role for ceramide in the regulation of mouse epidermal keratinocyte proliferation and differentiation

The Journal of Investigative Dermatology
E M JungW B Bollag

Abstract

We have previously determined that sustained phospholipase D (PLD) activation is associated with differentiation induction in primary mouse epidermal keratinocytes. We therefore investigated the effect of two bacterial PLD on keratinocyte proliferation and differentiation. We found that Streptomyces sp. PLD was much less potent at inhibiting proliferation than S. chromofuscus PLD, with a half-maximal inhibitory concentration of 0.05 versus less than 0.001 IU per ml for S. chromofuscus PLD. Similarly, S. chromofuscus PLD stimulated transglutaminase activity more effectively and potently than S. sp. PLD. When we examined the formation of products by the two PLD, we found that the S. sp. PLD showed higher activity at all concentrations. Whereas the PLD from S. sp. is relatively inactive on sphingomyelin, S. chromofuscus PLD is known to hydrolyze both glycerophospholipids and sphingomyelin. Based on recent data indicating a role for ceramide in regulating cell growth and differentiation, we hypothesized that the ability of S. chromofuscus PLD to hydrolyze sphingomyelin might underlie its greater potency. Therefore, we examined the effect of exogenous sphingomyelinase and synthetic ceramides on DNA synthesis. We found that sphingomy...Continue Reading

References

Nov 1, 1979·The Journal of Investigative Dermatology·P M EliasR J White
Dec 1, 1992·Experimental Dermatology·S Inohara
Oct 1, 1992·Trends in Biochemical Sciences·M Liscovitch
Aug 1, 1992·The Journal of Clinical Investigation·L C WoodK R Feingold
Jan 1, 1989·The American Journal of Physiology·C M IsalesP Q Barrett
Oct 1, 1995·Endocrinology·Y Zhang, R Kolesnick
Jul 1, 1994·The Journal of Cell Biology·R A Heller, M Krönke
May 6, 1994·Cell·M Liscovitch, L C Cantley
May 1, 1996·The Journal of Investigative Dermatology·K C Madison, E J Howard

❮ Previous
Next ❯

Citations

Mar 31, 2007·Skin Pharmacology and Physiology·E HoubenV Rogiers
Jul 18, 2008·Experimental Dermatology·György ParaghGerd Schmitz
Mar 6, 2012·Chemistry and Physics of Lipids·Shimon Gatt, Arie Dagan
Dec 19, 2013·PloS One·Luciana K Rosselli-MuraiRogerio M Castilho
Mar 29, 2003·The Journal of Pharmacology and Experimental Therapeutics·Stephen HsuGeorge Schuster
Mar 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tarek A TahaLina M Obeid
Feb 13, 1999·The Journal of Biological Chemistry·R D GrinerW B Bollag
Jul 16, 2008·The Journal of Biological Chemistry·Francisco Leocata NietoNorma B Sterin-Speziale
Feb 1, 2000·Trends in Cell Biology·Y A Hannun, C Luberto
May 30, 2001·Molecular and Cellular Endocrinology·W Bollinger Bollag, R J Bollag
Feb 26, 2000·The Journal of Investigative Dermatology·K HanleyK R Feingold
Dec 13, 2006·Biochimica Et Biophysica Acta·Tarek A TahaLina M Obeid

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.