PMID: 107971Mar 29, 1979

A potential role for phospholipids in the regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase in cultured C-6 glial cells. Effects of N,N-dimethylethanolamine

Biochimica Et Biophysica Acta
R S Finkel, J J Volpe

Abstract

The relation of the activity of the microsomal enzyme, 3-hydroxy-3-methylglutaryl coenzyme A reductase, to cellular phospholipid composition was studied in C-6 glial cells. Phospholipid composition was perturbed by growth of cells in the naturally occurring amino alcohol, N,N-dimethylethanolamine. After growth of C-6 glia in 5 mM N,N-dimethylethanolamine for 24 h, reductase activity was diminished by 50%. A similar diminution in cholesterol synthesis was observed. This effect was not accompanied by any parallel change in cell growth, DNA synthesis, protein synthesis, fatty acid synthetase activity, or microsomal NADPH-cytochrome c reductase activity. The inhibition of reductase activity by N,N-dimethylethanolamine was prevented by the addition of equimolar concentrations of choline to the culture medium and, also, could be reversed completely by removal of N,N-dimethylethanolamine from the culture medium. The effect of N,N-dimethylethanolamine on reductase was associated with the formation of phosphatidyl-N,N-dimethylethanolamine which accumulated primarily at the expense of phosphatidylcholine and, after 24 h, accounted for 27% of total phospholipid phosphorus. The data demonstrate that incorporation of N,N-dimethylethanolamin...Continue Reading

References

Nov 1, 1976·Archives of Biochemistry and Biophysics·M L BlankF Snyder
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·V H EngelhardM Glaser
Mar 1, 1972·Journal of Neurochemistry·J P ZanettaI G Morgan
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M S BrownJ L Goldstein
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M GlaserP R Vagelos
Feb 1, 1970·Archives of Biochemistry and Biophysics·D I Wilkinson
Mar 1, 1965·Archives of Biochemistry and Biophysics·K D MUNKRES, F M RICHARDS

Citations

Oct 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P MondolaM Bifulco
Jul 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P Mondola
Apr 1, 1982·Journal of Neurochemistry·J J Volpe, K A Obert
Mar 31, 2006·The British Journal of Nutrition·Anne MoriseDominique Hermier
Sep 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R T Holzbach
Jul 18, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·F CourtoisE Levy

Related Concepts

Epicholesterol
Choline Hydroxide
DNA, Double-Stranded
Ethanolamines
Hydroxymethylglutaryl-CoA reductase
NADPH-Ferrihemoprotein Reductase
Nerve Tissue Proteins
Neuroglia
Phospholipids
Fatty Acid Synthases

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