A potential role for the Src-like adapter protein SLAP-2 in signaling by the colony stimulating factor-1 receptor

The FEBS Journal
Gael A ManesGlen M Scholz

Abstract

The development of macrophages from myeloid progenitor cells is primarily controlled by the growth factor colony stimulating factor-1 (CSF-1) and its cognate receptor, a transmembrane tyrosine kinase encoded by the c-Fms proto-oncogene. The CSF-1 receptor exerts its biological effects on cells via a range of signaling proteins including Erk1/2 and Akt. Here we have investigated the potential involvement of the Src-like adapter protein (SLAP-2) in signaling by the CSF-1 receptor in mouse bone marrow-derived macrophages. RT-PCR analysis revealed constitutive expression of the SLAP-2 gene in bone marrow macrophages. Surprisingly, co-immunoprecipitation and GST binding experiments demonstrated that the CSF-1 receptor could bind to SLAP-2 in a ligand-independent manner. Furthermore, the binding of SLAP-2 to the CSF-1 receptor involved multiple domains of SLAP-2. SLAP-2 also bound c-Cbl, with the interaction being mediated, at least in part, by the unique C-terminal domain of SLAP-2. Overexpression of SLAP-2 in bone marrow macrophages partially suppressed the CSF-1-induced tyrosine phosphorylation and/or expression level of a approximately 80 kDa protein without affecting CSF-1-induced global tyrosine phosphorylation, or activation o...Continue Reading

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Citations

Nov 17, 2009·Immunological Reviews·Leonard L DragoneArthur Weiss
Mar 13, 2010·Journal of Cellular Biochemistry·Hyun-Ju KimSteven L Teitelbaum
Sep 5, 2015·Mediators of Inflammation·Nikolett MartonGyörgy Nagy
Mar 17, 2015·Cellular and Molecular Life Sciences : CMLS·Julhash U KaziLars Rönnstrand
Mar 14, 2007·The Journal of Biological Chemistry·Benjamin PakutsC Jane McGlade
Jan 12, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Larissa M LiontosC Jane McGlade
Feb 23, 2021·Journal of Molecular Biology·Leanne E Wybenga-GrootC Jane McGlade

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