A potential role of somatostatin and its receptor SSTR4 in the migration of hepatic oval cells

Laboratory Investigation; a Journal of Technical Methods and Pathology
Youngmi JungB E Petersen

Abstract

Somatostatin (SST) is a regulatory peptide that activates G protein-coupled receptors comprised of five members (somatostatin receptors (SSTRs) 1-5). Despite the broad use of SST and its analogs in clinical practice, the spectrum of SST activities has been incompletely defined. Recently, it has been demonstrated that SST can be a chemoattractant for hematopoietic precursor cells. Since hepatic oval cells (HOCs) share common characteristics with hematopoietic stem cells, we hypothesized that SST could act as a chemoattractant for HOCs by stimulating SSTRs. Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot assay revealed an increased expression of SST in the 2-acetyl-aminofluorene (2AAF)/partial hepatectomy (PHx) HOC induction model. Immunohistochemical staining showed the expression of SST in 2AAF/PHx-treated rat liver, as compared to normal liver. Proliferation and migration assays demonstrated that the increase of SST was related to migration of HOCs, but not their proliferation. RT-PCR and quantitative real-time PCR showed that SSTR4 was preferentially expressed by HOCs. Western blot assay and immunohistochemical staining confirmed the expression of SSTR4 by HOCs. In addition, pretreatment with anti-SS...Continue Reading

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Citations

Jun 15, 2007·Journal of Cellular Physiology·George K Michalopoulos
Oct 1, 2013·Hepatology International·Sihyung WangYoungmi Jung
Nov 22, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Youngmi JungBryon E Petersen
Jul 17, 2013·Asia-Pacific Psychiatry : Official Journal of the Pacific Rim College of Psychiatrists·Rongrong ZhaoWei Hao
Dec 5, 2018·European Journal of Medicinal Chemistry·Arvind NegiPaul V Murphy

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