Nov 25, 1977

A potential screening technique for neurotransmitters in the CNS: model studies in the cat spinal cord

Brain Research
W R Woodward, S Lindström

Abstract

This paper describes a potential screening technique for neurotransmitters in the CNS. The method uses the injection of small volumes of high specific activity radioactive transmitter precursor substances into regions of physiologically identified neuronal cell bodies, and the later identification of the substances transported down axons to target tissues. Experiments were performed in motoneurons in the cat spinal cords to test the feasibility of method. Tritiated choline, glutamate, tyramine and tryptophan were pressure-injected into the ventral horn using glass micropipettes that were adapted to allow similtaneous physiological recording and injection. Only tritiated acetylcholine, two unidentified choline metabolites and a small amount of choline were found in the motor axons. The acetylcholine migrated at a rate of greater than 24mm/day and the movement was blocked by colchicine. The spread of isotope from the injection site was measured by a direct chemical method and by autoradiography, and was found that isotope spread1-2 mm from the injection site. One unexpected finding in the autoradiographs was that the motoneurons were selectively labelled following choline injections.

  • References12
  • Citations15

References

  • References12
  • Citations15

Citations

Mentioned in this Paper

Axonal Transport
Motor Neurons
Axon
Neurohormones
Autoradiography
Choline Hydroxide
Acetylcholine Sulfate (1: 1)
Spinal Cord
Ganglia, Spinal
Choline Deficiency

About this Paper

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