A practical approach estimating etiologic agents using real-time PCR in pediatric inpatients with community-acquired pneumonia

Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy
Takafumi OkadaKimiko Ubukata

Abstract

To evaluate pathogens in pediatric inpatients with community-acquired pneumonia (CAP), an Acute Respiratory Diseases Study Group organized by ten Japanese medical institutions devised a rapid, reliable process based on real-time PCR results in nasopharyngeal swab samples plus admission blood test results. From April 2008 to April 2009, we enrolled 903 children with CAP based on chest radiographs and clinical findings who were hospitalized within 5 days of onset. Comprehensive real-time PCR was used to detect 6 bacteria and 11 respiratory viruses. The swab specimens also were used for bacterial cultures. After initial determination of presence or absence of viral and mycoplasmal infections, significant bacterial contributions were defined by bacterial identification, clinical efficacy of antimicrobial agent, and reference to blood test results. Children were stratified by age: below 1 year, 1 year, 2-5 years, or at least 6 years old. Among patients studied, 34.4 % were diagnosed with viral infection; 21.8 %, bacterial infection; 17.5 %, viral/bacterial co-infection; 5.9 %, mycoplasmal infection; 0.3 %, mycoplasmal/bacterial co-infection; and 1.7 %, viral/mycoplasmal co-infection. The remaining 18.4 % had unknown pathogens. Purel...Continue Reading

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Citations

Dec 18, 2013·Acta Paediatrica·Elena ChiappiniMaurizio de Martino
Jan 24, 2015·Respirology : Official Journal of the Asian Pacific Society of Respirology·Min WangYi Shi
Oct 31, 2012·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Miyuki MorozumiKimiko Ubukata
Oct 8, 2016·European Journal of Pediatrics·Tatyana V SpichakMatti O Korppi
Apr 17, 2015·FEMS Microbiology Letters·Jaelle C BrealeyKeith J Chappell
Feb 28, 2017·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Hiroshi SakataUNKNOWN World's First Oral Carbapenem Study Group
Sep 14, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Takafumi OkadaKimiko Ubukata
Jan 12, 2016·Paediatrics and International Child Health·Yusuf AydemirMehmet Özdemir
May 4, 2021·The Laryngoscope·Shoichi Sawada, Shigenori Matsubara
Jun 16, 2021·Population Health Metrics·Emily K JohnsonSpencer L James

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