A practical guide to small angle X-ray scattering (SAXS) of flexible and intrinsically disordered proteins

FEBS Letters
Alexey G Kikhney, Dmitri I Svergun

Abstract

Small-angle X-ray scattering (SAXS) is a biophysical method to study the overall shape and structural transitions of biological macromolecules in solution. SAXS provides low resolution information on the shape, conformation and assembly state of proteins, nucleic acids and various macromolecular complexes. The technique also offers powerful means for the quantitative analysis of flexible systems, including intrinsically disordered proteins (IDPs). Here, the basic principles of SAXS are presented, and profits and pitfalls of the characterization of multidomain flexible proteins and IDPs using SAXS are discussed from the practical point of view. Examples of the synergistic use of SAXS with high resolution methods like X-ray crystallography and nuclear magnetic resonance (NMR), as well as other experimental and in silico techniques to characterize completely, or partially unstructured proteins, are presented.

References

Jan 13, 2000·Biochemical and Biophysical Research Communications·V N UverskyV M Abramov
May 31, 2001·Journal of Molecular Graphics & Modelling·A K DunkerZ Obradovic
Oct 9, 2002·Trends in Biochemical Sciences·Peter Tompa
Feb 22, 2003·Journal of Molecular Biology·David P Goldenberg
Feb 26, 2004·Biochemistry·Huan-Xiang Zhou
May 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Richard L KingstonBrian W Matthews
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jonathan E KohnKevin W Plaxco
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Nicholas C Fitzkee, George D Rose
Sep 8, 2004·Journal of Molecular Biology·Larissa A MunishkinaVladimir N Uversky
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·H Jane Dyson, Peter E Wright
Jun 1, 2005·Biophysical Journal·Maxim V Petoukhov, Dmitri I Svergun
Jul 12, 2005·Structure·Feng DingNikolay V Dokholyan
Jul 28, 2005·The Journal of Biological Chemistry·Gen HeAnne George
Sep 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Abhishek K JhaTobin R Sosnick
Nov 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Pau BernadóMartin Blackledge
Nov 25, 2005·Journal of the American Chemical Society·Alexander GrishaevAd Bax
Apr 7, 2006·Protein Science : a Publication of the Protein Society·Marco NardiniMartino Bolognesi
Jun 8, 2006·Annual Review of Biochemistry·Fabrizio Chiti, Christopher M Dobson
Jul 27, 2006·Biochemistry·Luis Maurício T R LimaJerson L Silva
Dec 1, 1994·Biophysical Chemistry·P CalmettesJ C Smith
Apr 7, 2007·Journal of the American Chemical Society·Pau BernadóDmitri I Svergun
May 29, 2007·Molecular Cell·Scarlet S ShellRichard D Kolodner
Jul 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Henning TidowAlan R Fersht
Jul 31, 2007·Nature Structural & Molecular Biology·Jennifer M R BakerJulie D Forman-Kay
Dec 1, 2007·Journal of the American Chemical Society·Martin C Stumpe, Helmut Grubmüller
Dec 7, 2007·Trends in Biochemical Sciences·Peter Tompa, Monika Fuxreiter
Apr 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mark WellsAlan R Fersht
Jun 25, 2008·Annual Review of Biophysics·Vladimir N UverskyA Keith Dunker
Sep 9, 2008·Journal of Molecular Biology·Dong-Pyo HongVladimir N Uversky
Oct 28, 2008·Current Opinion in Structural Biology·A Keith DunkerJoel L Sussman
Dec 18, 2008·Proteins·Francesca PaolettiDoriano Lamba
Mar 14, 2009·The Journal of Biological Chemistry·Ivano BertiniDmitri I Svergun

❮ Previous
Next ❯

Citations

Feb 18, 2016·Biochemistry·Julien PérardEve de Rosny
Dec 19, 2015·Biochemistry·Bushra HusainJames L Cole
May 10, 2016·Biochimica Et Biophysica Acta. General Subjects·Zehra SayersInes Karmous
Aug 16, 2016·International Journal of Molecular Sciences·Marie-Laurence TremblayJan K Rainey
Sep 27, 2016·Frontiers in Molecular Biosciences·Martina PalaminiFederico Forneris
Nov 3, 2016·The Journal of Physical Chemistry. B·Anna G BaldwinJenifer C Braley
Dec 3, 2016·Nucleic Acids Research·Arka ChakrabortyMaria Solà
Apr 5, 2017·Annual Review of Physical Chemistry·Hosung KiHyotcherl Ihee
Jul 14, 2017·The Journal of Physical Chemistry. a·David Ormrod MorleyMark Wilson
Dec 6, 2017·International Journal of Molecular Sciences·John Oliver NealonLiam James McGuffin
Dec 3, 2017·Scientific Reports·Xiaofeng LiTitus J Boggon
Dec 1, 2017·European Journal of Mass Spectrometry·Yelena YefremovaMichael O Glocker
Jun 2, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Dolev RimmermanLin X Chen
Aug 4, 2018·Advanced Materials·Sivaraman SubramanianFrank Vollmer
Dec 18, 2018·PLoS Computational Biology·Wojciech PotrzebowskiIngemar Andre
Dec 24, 2018·RNA·Meghan S WardenSteven M Pascal
Jul 14, 2017·The Biochemical Journal·Lasse StabyKaren Skriver
Oct 24, 2019·Protein Science : a Publication of the Protein Society·Peter S MillardBirthe B Kragelund
Feb 29, 2020·PLoS Computational Biology·Lenka StejskalJoe Grove
Feb 28, 2020·Membranes·Yousef Alqaheem, Abdulaziz A Alomair
Nov 7, 2019·Scientific Reports·Carlos Rullán-LindAbel Baerga-Ortiz
Jun 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ivan PeranDaniel P Raleigh
Feb 23, 2020·Nucleic Acids Research·Robert C MonsenJohn O Trent
Aug 18, 2018·Scientific Reports·Bianca PérezBirgit Schiøtt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The International Journal of Biochemistry & Cell Biology
Maxim V Petoukhov, Dmitri I Svergun
Journal of the American Chemical Society
Pau BernadóDmitri I Svergun
© 2021 Meta ULC. All rights reserved