A practical method for patterning lumens through ECM hydrogels via viscous finger patterning.

Journal of Laboratory Automation
Lauren L BischelDavid J Beebe

Abstract

Extracellular matrix (ECM) hydrogels with patterned lumens have been used as a framework to generate more physiologically relevant models of tissues, such as vessels and mammary ducts, for biological investigations. However, these models have not found widespread use in research labs or in high-throughput screening applications in large part because the basic methods for generating the lumen structures are generally cumbersome and slow. Here we present viscous finger patterning, a technique to generate lumens through ECM hydrogels in microchannels that can be accomplished using manual or automated pipetting. Passive pumping is used to flow culture media through an unpolymerized hydrogel, creating a lumen through the hydrogel that is subsequently polymerized. Viscous finger patterning takes advantage of viscous fingering, the fluid dynamics phenomenon where a less viscous fluid will flow through and displace a more viscous fluid. We have characterized the technique and used it to create a variety of channel geometries and ECM hydrogel compositions, as well as for the generation of lumens surrounded by multiple hydrogel layers. Because viscous finger patterning can be performed with automated liquid handling systems, high-through...Continue Reading

References

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Citations

Jul 16, 2014·Advanced Drug Delivery Reviews·Kyung Eun Sung, David J Beebe
Mar 20, 2014·Current Opinion in Chemical Engineering·Quinton Smith, Sharon Gerecht
Jan 22, 2015·BMC Cancer·Lauren L BischelKyung E Sung
Oct 27, 2016·Expert Opinion on Drug Discovery·Mohammad A KaisarLuca Cucullo
Jul 9, 2013·Journal of Laboratory Automation·Edmond W K Young
Feb 10, 2017·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Ehsan AkbariJonathan W Song
May 17, 2018·Angiogenesis·Patrycja Nowak-SliwinskaArjan W Griffioen
Nov 27, 2015·Advanced Healthcare Materials·José A Jiménez-TorresDavid J Beebe
May 3, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Lauren L BischelDavid J Beebe
Jun 24, 2017·Journal of the Royal Society, Interface·Hsieh-Fu TsaiGang Bao
Sep 15, 2018·Journal of Biological Engineering·Jose YesteRosa Villa
Feb 12, 2019·Biofabrication·Alisa Morss ClyneAndrés Díaz Lantada
Aug 1, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lauren L BischelDavid J Beebe
May 7, 2013·Lab on a Chip·Anja van de Stolpe, Jaap den Toonder
Sep 30, 2020·Expert Opinion on Drug Discovery·Carley OrtChristopher Moraes
Jul 3, 2019·APL Bioengineering·Mees N S de GraafValeria V Orlova
Feb 23, 2020·Bioengineering·Andreas M A O Pollet, Jaap M J den Toonder
Sep 8, 2020·Advanced Healthcare Materials·Joe Tien, Yoseph W Dance
Aug 7, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Halston E DealMichael A Daniele
Jan 8, 2020·Micromachines·Andreas M A O PolletJaap M J den Toonder
Aug 8, 2020·Chemical Society Reviews·María Virumbrales-MuñozDavid J Beebe
Nov 12, 2020·American Journal of Physiology. Cell Physiology·Savannah R MosesJonathan W Song
Jan 6, 2021·Tissue Engineering. Part a·Jonathan KulwatnoKristen L Mills
Mar 20, 2021·ACS Applied Materials & Interfaces·Daniel VeraElena Martinez
Mar 18, 2021·Cardiovascular Research·Valentina PaloschiLars Maegdefessel
May 15, 2021·Frontiers in Immunology·Isaac M RichardsonLaurel E Hind
Jul 20, 2021·ACS Biomaterials Science & Engineering·Kristen L HaywardBoyang Zhang
Jul 22, 2021·Cell Reports·Janna C NawrothKatia C Karalis
Jul 24, 2021·Advanced Healthcare Materials·Hyeonji YuJungwook Kim
Aug 7, 2021·Micromachines·Kena SongJinghua Li

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Software Mentioned

PixeLINK Capture
Osirix
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