A pre-docking role for microtubules in insulin-stimulated glucose transporter 4 translocation

The FEBS Journal
Yu ChenTao Xu

Abstract

Insulin stimulates glucose uptake by inducing translocation of glucose transporter 4 (GLUT4) from intracellular resides to the plasma membrane. How GLUT4 storage vesicles are translocated from the cellular interior to the plasma membrane remains to be elucidated. In the present study, intracellular transport of GLUT4 storage vesicles and the kinetics of their docking at the plasma membrane were comprehensively investigated at single vesicle level in control and microtubule-disrupted 3T3-L1 adipocytes by time-lapse total internal reflection fluorescence microscopy. It is demonstrated that microtubule disruption substantially inhibited insulin-stimulated GLUT4 translocation. Detailed analysis reveals that microtubule disruption blocked the recruitment of GLUT4 storage vesicles to underneath the plasma membrane and abolished the docking of them at the plasma membrane. These data suggest that transport of GLUT4 storage vesicles to the plasma membrane takes place along microtubules and that this transport is obligatory for insulin-stimulated GLUT4 translocation.

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Citations

Mar 6, 2013·European Journal of Pharmacology·Luisa Helena CazarolliFátima Regina Mena Barreto Silva
May 24, 2012·Nature Reviews. Molecular Cell Biology·Dara Leto, Alan R Saltiel
Sep 21, 2012·The Journal of Biological Chemistry·Paul LanglaisLawrence J Mandarino
Jul 17, 2009·Molecular Biology of the Cell·Jamie A LopezWilliam E Hughes
Aug 22, 2012·The Journal of Cell Biology·Yu ChenJennifer Lippincott-Schwartz
Sep 22, 2012·Molecular and Cellular Endocrinology·Li-Zhong LiuPeter C Y Tong
Oct 9, 2015·Journal of Cellular Biochemistry·Marisa Jádna Silva FredericoFátima Regina Mena Barreto Silva
Dec 17, 2008·Chemico-biological Interactions·Luisa Helena CazarolliFátima Regina Mena Barreto Silva
Sep 14, 2013·Small GTPases·Yu Chen, Jennifer Lippincott-Schwartz
Dec 10, 2013·The International Journal of Biochemistry & Cell Biology·Allisson Jhonatan Gomes CastroFátima Regina Mena Barreto Silva
May 28, 2017·Molecular & Cellular Proteomics : MCP·Rikke KrusePaul R Langlais
Jun 16, 2011·Physiological Genomics·Evert M van SchothorstJaap Keijer
Jan 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·SaiSantosh Babu KomakulaShashi Singh
Sep 1, 2021·Trends in Endocrinology and Metabolism : TEM·Skylar R Batty, Paul R Langlais

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