PMID: 6439433Oct 1, 1984

A prealbumin variant with an increased affinity for T4 and reverse-T3

Clinical Endocrinology
M R LallozR L Himsworth

Abstract

A euthyroid adult female (LC) was found to have persistently raised concentrations of total T4 (159 nmol/l) and rT3 (500 pmol/l) in her serum in association with a normal T3 (2.1 nmol/l). Serum concentrations of all three T4-binding proteins were within normal limits. A variant prealbumin with an increased affinity for T4 was found to be responsible for the raised serum level of T4. Unlike normal prealbumin, the variant also bound appreciable amounts of rT3. The affinity constant for T4 binding to prealbumin LC was 5.5 X 10(8) l/mole which is sevenfold higher than that obtained for normal prealbumin (8.5 X 10(7) l/mole). The affinity constant for the binding of rT3 to prealbumin LC was 2.0 X 10(6) l/mole while that for the normal protein was unmeasurable by our method. The T4-binding capacity of prealbumin LC in serum was within the normal range indicating that there is no new additional T4-binding site on the protein. Prealbumin LC has the same molecular size as the normal protein, hence it is likely that the tetrameric structure has been preserved. The electrophoretic mobility of prealbumin LC was normal indicating no alteration in charge. It is postulated that the increased affinity for T4 (and presumably for rT3) results fr...Continue Reading

References

Mar 1, 1979·The Journal of Endocrinology·P G ByfieldR L Himsworth
Jan 1, 1978·Methods in Enzymology·F W Dahlquist
Dec 26, 1978·Biochemistry·G Irace, H Edelhoch
Jun 15, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Hüfner, M Grussendorf
Aug 1, 1981·The Journal of Clinical Endocrinology and Metabolism·J R StockigtP Taft
Mar 18, 1982·The New England Journal of Medicine·M RuizS H Ingbar
Jan 1, 1983·Clinical Endocrinology·M R LallozR L Himsworth
Aug 1, 1961·American Journal of Clinical Pathology·K E ELZINGAW H BEIERWALTES

Citations

Jan 1, 1992·Thyroid : Official Journal of the American Thyroid Association·B J ScrimshawP M George
Jan 1, 1992·Thyroid : Official Journal of the American Thyroid Association·L Bartalena, J Robbins
Aug 1, 1990·Clinical Endocrinology·M T AkbariD B Ramsden
Apr 1, 1985·Australian and New Zealand Journal of Medicine·J R Stockigt, J W Barlow
Jan 1, 1986·International Journal of Health Services : Planning, Administration, Evaluation·R Hubbard
May 1, 1986·Annals of Clinical Biochemistry·C J Pearce, P G Byfield

Related Concepts

Electrophoresis, Paper
Prealbumin
Plasma Protein Binding Capacity
Plasma Albumin
Novothyral
Thyroxine-Binding Proteins
Cytomel
Reverse Triiodothyronine

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