A prediction of cell differentiation and proliferation within a collagen-glycosaminoglycan scaffold subjected to mechanical strain and perfusive fluid flow

Journal of Biomechanics
A J F StopsP E McHugh

Abstract

Mesenchymal stem cell (MSC) differentiation can be influenced by biophysical stimuli imparted by the host scaffold. Yet, causal relationships linking scaffold strain magnitudes and inlet fluid velocities to specific cell responses are thus far underdeveloped. This investigation attempted to simulate cell responses in a collagen-glycosaminoglycan (CG) scaffold within a bioreactor. CG scaffold deformation was simulated using micro-computed tomography (CT) and an in-house finite element solver (FEEBE/linear). Similarly, the internal fluid velocities were simulated using the afore-mentioned microCT dataset with a computational fluid dynamics solver (ANSYS/CFX). From the ensuing cell-level mechanics, albeit octahedral shear strain or fluid velocity, the proliferation and differentiation of the representative cells were predicted from deterministic functions. Cell proliferation patterns concurred with previous experiments. MSC differentiation was dependent on the level of CG scaffold strain and the inlet fluid velocity. Furthermore, MSC differentiation patterns indicated that specific combinations of scaffold strains and inlet fluid flows cause phenotype assemblies dominated by single cell types. Further to typical laboratory procedu...Continue Reading

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Citations

Sep 25, 2010·Biomechanics and Modeling in Mechanobiology·Clara Sandino, Damien Lacroix
Sep 4, 2012·Journal of Biomechanical Engineering·Emma C MoranJessica L Sparks
Sep 2, 2014·Advanced Drug Delivery Reviews·Maureen E Lynch, Claudia Fischbach
Sep 23, 2014·Advanced Drug Delivery Reviews·Marc A Fernandez-YagueManus J Biggs
May 22, 2016·Computer Methods and Programs in Biomedicine·Seyed Jamaleddin Mousavi, Mohamed Hamdy Doweidar
Aug 1, 2015·Biomechanics and Modeling in Mechanobiology·Feihu ZhaoLaoise M McNamara
May 2, 2015·PloS One·Seyed Jamaleddin Mousavi, Mohamed Hamdy Doweidar
Dec 14, 2017·Applied Biochemistry and Biotechnology·Birru BhaskarGwendolen C Reilly
Jun 1, 2016·Iranian Journal of Biotechnology·Maliheh YaghoobiSeyyed Mohammad Mousavi
Aug 18, 2018·Medical & Biological Engineering & Computing·Xianbin ZhangHåvard J Haugen
Sep 19, 2019·Biotechnology and Bioengineering·Mikhael Hadida, David Marchat
Sep 24, 2020·BioMed Research International·Amr AlraiesRyan Moseley
Feb 4, 2011·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·M S ThompsonG N Duda
Aug 6, 2017·Biomechanics and Modeling in Mechanobiology·Feihu ZhaoLaoise M McNamara
Mar 31, 2021·Computer Methods and Programs in Biomedicine·Mengguang FuGuimei Lin

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