A predominant and virulent Legionella pneumophila serogroup 1 strain detected in isolates from patients and water in Queensland, Australia, by an amplified fragment length polymorphism protocol and virulence gene-based PCR assays

Journal of Clinical Microbiology
Bixing HuangJohn Savill

Abstract

In epidemiological investigations of community legionellosis outbreaks, knowledge of the prevalence, distribution, and clinical significance (virulence) of environmental Legionella isolates is crucial for interpretation of the molecular subtyping results. To obtain such information for Legionella pneumophila serogroup 1 isolates, we used the standardized amplified fragment length polymorphism (AFLP) protocol of the European Working Group on Legionella Infection to subtype L. pneumophila SG1 isolates obtained from patients and water sources in Queensland, Australia. An AFLP genotype, termed AF1, was predominant in isolates from both patients (40.5%) and water (49.0%). The second most common AFLP genotype found in water isolates was AF16 (36.5%), but this genotype was not identified in the patient isolates. When virulence gene-based PCR assays for lvh and rtxA genes were applied to the isolates from patients and water, nearly all (65 of 66) AF1 strains had both virulence genes, lvh and rtxA. In contrast, neither the lvh nor the rtxA gene was found in the AF16 strains, except for one isolate with the rtxA gene. It appears that this may explain the failure to find this genotype in the isolates from patients even though it may be co...Continue Reading

References

Mar 1, 1988·The Journal of Infectious Diseases·E DournonR M McKinney
Jan 1, 1981·Applied and Environmental Microbiology·C B FliermansD H Pope
Feb 1, 1996·Trends in Microbiology·S C WinansP J Christie
Jul 11, 1998·Australian and New Zealand Journal of Public Health·T C HeathA G Capon
Feb 27, 1999·Current Opinion in Microbiology·J P Vogel, R R Isberg
Sep 17, 1999·Journal of Clinical Microbiology·P H SavelkoulJ A Lenstra
Sep 17, 1999·Journal of Clinical Microbiology·J C Montanaro-PunzengruberG L Gilbert
Dec 30, 1999·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·M S Rangel-FraustoL A Herwaldt
Jun 10, 2000·Microbiology·S L CirilloJ D Cirillo
Oct 6, 2000·Annual Review of Microbiology·M S Swanson, B K Hammer
Dec 19, 2000·Infection and Immunity·S L CirilloJ D Cirillo
Feb 22, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Norman K. FryTimothy G. Harrison
Mar 30, 2002·Journal of Clinical Microbiology·Mustapha M SamrakandiJeffrey D Cirillo
Jul 5, 2002·Clinical Microbiology Reviews·Barry S FieldsRichard E Besser
Nov 5, 2002·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·N K FryT G Harrison
Jul 5, 2003·Journal of Clinical Microbiology·Helena AurellSophie Jarraud
Jan 13, 2004·Journal of Clinical Microbiology·Anne DoleansSophie Jarraud

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Citations

Dec 14, 2011·International Journal of Environmental Health Research·K FragouA Vantarakis
Jan 5, 2006·Applied and Environmental Microbiology·Bart A Wullings, Dick van der Kooij
Jan 5, 2006·Journal of Clinical Microbiology·Nathalie van der Mee-MarquetRoland Quentin
Mar 1, 2014·Epidemiology and Infection·R M A GrahamA V Jennison
Feb 19, 2013·Applied and Environmental Microbiology·Jingrang LuNicholas J Ashbolt
May 23, 2006·Microbiology and Immunology·Hsun-Pi SuTung-Ching Chung
Aug 18, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·N K FryT G Harrison

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