A preliminary examination of the role of NFAT 3 in human skin, cultured keratocytes and dermal fibroblasts

Journal of Cutaneous Pathology
Wael I Al-DarajiBernhard Zelger

Abstract

Ciclosporin A (CsA) is widely utilized for the treatment of inflammatory skin diseases such as psoriasis.The therapeutic effects of CsA are thought to be mediated via its immunosuppressive action on infiltrating lymphocytes in skin lesions. CsA and tacrolimus block T cell activation by inhibiting the phosphatase calcineurin and preventing translocation from the cytoplasm to the nucleus of the transcription factor Nuclear Factor of Activated T cells (NFAT). RT-PCR and Western Analysis were used to investigate the presence of NFAT-3 mRNA and protein in human keratocytes. Tissue culture of human keratocytes and immunostaining of cells on coverslips and confocal microscopy were used to assess the degree of nuclear localisation of NFAT-3 in cultured cells. Keratome biopsies were taken from patients with psoriasis (lesional and non-lesional skin) and normal skin and immunohistochemistry was used to assess the NFAT-3 localisation in these biopsies using a well characterized anti-NFAT-3 antibody. The NFAT-3 mRNA and protein expression was demonstrated using RT-PCR and Western blotting. The expression of NFAT-3 in human keratocytes and response to different agonists provides perhaps a unique opportunity to examine the regulation, subcel...Continue Reading

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Citations

Dec 21, 2010·The British Journal of Dermatology·D F FiorentinoU N Sundram
Dec 24, 2010·Journal of Cutaneous Pathology·Timothy H McCalmont
Oct 4, 2013·Pharmacogenomics·Rocío Prieto-PérezFrancisco Abad-Santos

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