A preliminary study of side population cells in human gastric cancer cell line HGC-27

Annals of Transplantation : Quarterly of the Polish Transplantation Society
Ganglong GaoXueli Zhang

Abstract

Cancer stem cell-like side population (SP) cells, which may be responsible for recurrence, tumor metastasis, and resistance to cancer therapy, have been identified and characterized in several types of cell lines from gastric cancer. However, there is no report on isolation of SP cells from human gastric cancer cell line HGC-27. This study aims to analyze the proportion of SP cells in HGC-27 cell line, differentiate SP from non-side population (NSP) cells, and determine whether the SP cells have certain biological properties of stem cells. (1) HGC-27 suspension was prepared and stained with Hoechst33342 and PI for flow cytometric isolation of SP (2). Differences in proliferation and stemness-related gene expression profiles (CD133, CD44, OCT-4, MDR1, EpCAM, and ABCG2) between SP and NSP cells were detected by gastric formation assay and quantitative real-time PCR (3). Oncogenicity of SP and NSP cells was determined in nude mice in vivo. (1) SP cells accounted for 0.1-1.0% of HGC-27 cells, and decreased to 0% after verapamil inhibition. Using flow cytometry, we sorted 7.5×10⁵ SP cells and most HGC-27 cells were NSP cells (2). Gastric formation assay and MTT demonstrated that there was a significant difference in proliferation be...Continue Reading

Citations

Sep 28, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Hongyu ZhengGuohao Lu
Jul 23, 2016·International Journal of Molecular Sciences·Kaile ZhangXiaolan Fang
May 17, 2019·Journal of Cellular Biochemistry·Mahdieh BakhshiMonireh Hajimoradi
Oct 9, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Fatemeh B RassouliMorvarid Saeinasab

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