A preliminary study of the effect of curcumin on the expression of p53 protein in a human multiple myeloma cell line

Oncology Letters
Wei LiBaijun Fang

Abstract

Curcumin is an inexpensive, natural plant ingredient with protease inhibitor effects. The present study aimed to analyze the inhibitory effects of curcumin on the multiple myeloma (MM) RPMI 8226 cell line, and examine the underlying mechanism that promotes the apoptosis of RPMI 8226 cells. A growth curve was constructed in order to observe the relative growth velocity, and MTT was used to analyze the effect of different concentrations of curcumin on inhibiting the proliferation of the RPMI 8226 cells. The mRNA expression of the p53, Bax and MDM2 genes was detected using quantitative polymerase chain reaction. The expression of p53 protein in the MM RPMI 8226 cells following treatment with curcumin was detected by western blotting and ELISA. Curcumin inhibited the proliferation of the MM RPMI 8226 cells in a dose- and time-dependent manner. In the MM RPMI 8226 cells treated with curcumin, the expression of the p53 and Bax genes was upregulated, while the expression of the MDM2 gene was downregulated. p53 protein expression was higher in the curcumin experimental group compared with the control group. Subsequent to treatment with curcumin, the growth of the MM RPMI 8226 cell line was inhibited in a concentration- and time-depende...Continue Reading

References

Sep 1, 1999·British Journal of Haematology·H Avet-LoiseauR Bataille
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·B Li, Q P Dou
Aug 7, 2001·Seminars in Hematology·B BarlogieG Tricot
Dec 12, 2001·Seminars in Oncology·J Adams
May 9, 2003·The New England Journal of Medicine·J Anthony ChildUNKNOWN Medical Research Council Adult Leukaemia Working Party
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alok C BhartiBharat B Aggarwal
Jun 18, 2004·Cancer Investigation·Julian Adams, Michael Kauffman
Nov 23, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·G SongS D Bao
Nov 28, 2006·Trends in Molecular Medicine·Lyubomir T Vassilev
Jan 12, 2007·Leukemia·W J ChngR Fonseca
Jun 28, 2007·Medicina clínica·Ramón García-SanzJesús Fernando San Miguel
Jul 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Navneet DhillonRazelle Kurzrock
Sep 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sanjeev Shangary, Shaomeng Wang
Sep 4, 2008·Journal of Cancer Research and Clinical Oncology·Ivana von MetzlerOrhan Sezer
Jun 23, 2009·Lancet·Marc S RaabKenneth C Anderson
Dec 4, 2009·Journal of Clinical Pathology·Manujendra N SahaHong Chang
Jan 10, 2012·Biochemical Pharmacology·Sayantan JanaSnehasikta Swarnakar
Jan 9, 2015·Materia Socio-medica·Svetlana B KrstevskaSonja Genadieva-Stavric

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Citations

Apr 18, 2017·BMJ Case Reports·Abbas ZaidiJamie Cavenagh
Jan 10, 2019·Journal of Cellular Physiology·Keywan MortezaeeAmirhossein Sahebkar
Jun 20, 2019·Frontiers in Genetics·Faiz-Ul HassanChengjian Yang
May 31, 2018·Scientific Reports·Farid Ahmad SiddiquiMohammad Askandar Iqbal
Jul 23, 2019·European Journal of Medicinal Chemistry·Fanny PojeroMonica Notarbartolo
Nov 5, 2020·Recent Patents on Anti-cancer Drug Discovery·Saleh A AlmatroodiArshad Husain Rahmani

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Methods Mentioned

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PCR
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ELISA

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SPSS

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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