A preliminary study of the effect of growth hormone on mouse preimplantation embryo development in vitro

Gynecologic and Obstetric Investigation
P DrakakisA A Kiessling

Abstract

The role of growth hormone (GH) in follicular development, ovulation and embryo development is currently under reconsideration. In this study, we have tried to investigate the effect of GH on preimplantation development of mouse embryos in vitro. Zygotes and two-cell mouse embryos were cultured without (control) or with GH. For zygotes, the addition of 0.2 micrograms/ml of GH resulted in 77 +/- 1% of blastocysts formed and 66 +/- 3% rate of hatching (control 64 +/- 4 and 31 +/- 3%, p < 0.05 and p < 0.01, respectively). For two-cell embryos, the addition of 0.2 micrograms/ml of GH resulted in 87 +/- 2% of blastocysts formed and 60 +/- 4% hatching rate (control 76 +/- 4 and 47 +/- 5%, p < 0.05 for both). This positive effect of GH addition implies that the latter can support mouse preimplantation development in vitro and it suggests, along with its local action on the ovary and its possible effects, via the insulin-like growth factor system, on the tubal and uterine epithelium, a continuous role of this hormone in reproductive physiology from follicular maturation to embryonic development and, possibly, implantation.

Citations

Aug 7, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·M J Waters, P L Kaye
Apr 2, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·A KidsonM M Bevers
Oct 6, 2005·Reproductive Biology and Endocrinology : RB&E·Mohamed F M MitwallyMichael P Diamond
Jan 13, 2015·International Journal of Endocrinology·Kerry L Hull, Steve Harvey
Oct 25, 2016·Clinical Medicine Insights. Endocrinology and Diabetes·Jesús DevesaPablo Devesa
Jul 31, 2003·Reproduction in Domestic Animals = Zuchthygiene·E WolfF Sinowatz

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