PMID: 14761642Feb 6, 2004Paper

A preliminary study on the identification and distribution of epidermal stem cells in different degrees of burn wounds in scalded rats

Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns
Ju-lin XieHui-zhen Liang

Abstract

To investigate the distribution of epidermal stem cells (ESCs) in different degrees of burn wounds in scalded rats. Thirty-two Sprague-Dawley (SD) rats were employed in the study. First degree (I), shallow (shallow II) and deep partial thickness (deep II) and full thickness burn wounds (III) were created on the rat skin. Burn wound samples were harvested at 24 postburn hours (PBHs) from all the wounds and were processed to tissue slices. The tissue slices were stained by immunohistochemistry technique. The expression and distribution of ESCs in different degrees of burn wounds were observed with integrins alpha 2 beta 1 and keratin 10 (K10) as first antibodies. K10 positive cells were found to distribute in the strata spinosum, granulosum and lucidum in the first degree burn wound (I) with large amounts of integrins alpha 2 beta 1 positive cells in the residual basal layer and skin appendages (hair follicles) in shallow partial thickness burn wound (shallow II degree), and there were less integrins alpha 2 beta 1 positive cells in the remaining skin appendages in deep dermis in deep partial thickness burn wound (deep II degree). Finally, integrins alpha 2 beta 1 positive cells were sparsely found in the III degree burn wound. T...Continue Reading

Related Concepts

Burn Injury
Immunocytochemistry
Keratin-Associated Proteins
Colony-forming Unit
Rats, Holtzman
Integrin alpha2beta1
Keratin-10

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