A Presynaptic Function of Shank Protein in Drosophila

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Song WuYong Q Zhang

Abstract

Human genetic studies support that loss-of-function mutations in the SH3 domain and ankyrin repeat containing family proteins (SHANK1-3), the large synaptic scaffolding proteins enriched at the postsynaptic density of excitatory synapses, are causative for autism spectrum disorder and other neuropsychiatric disorders in humans. To better understand the in vivo functions of Shank and facilitate dissection of neuropathology associated with SHANK mutations in human, we generated multiple mutations in the Shank gene, the only member of the SHANK family in Drosophila melanogaster Both male and female Shank null mutants were fully viable and fertile with no apparent morphological or developmental defects. Expression analysis revealed apparent enrichment of Shank in the neuropils of the CNS. Specifically, Shank coexpressed with another PSD scaffold protein, Homer, in the calyx of mushroom bodies in the brain. Consistent with high expression in mushroom body calyces, Shank mutants show an abnormal calyx structure and reduced olfactory acuity. These morphological and functional phenotypes were fully rescued by pan-neuronal reexpression of Shank, and only partially rescued by presynaptic but no rescue by postsynaptic reexpression of Shan...Continue Reading

Citations

May 16, 2019·Disease Models & Mechanisms·Mireia Coll-TanéAnnette Schenck
Sep 5, 2019·Frontiers in Physiology·Paola Bellosta, Alessia Soldano
Jul 17, 2021·ELife·Shataakshi Dube O'NeilScott H Soderling

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