A primary cell culture of Drosophila postembryonic larval neuroblasts to study cell cycle and asymmetric division

European Journal of Cell Biology
Julian CeronFernando Moya

Abstract

Drosophila melanogaster is a key model system that has greatly contributed to the advance of developmental biology through its extensive and sophisticated genetics. Nevertheless, only a few in vitro approaches are available in Drosophila to complement genetic studies in order to better elucidate developmental mechanisms at the cellular and molecular level. Here we present a dissociated cell culture system generated from the optic lobes of Drosophila larval brain. This culture system makes it feasible to study the proliferative properties of Drosophila postembryonic Nbs by allowing BrdU pulse and chase assays, as well as detailed immunocytochemical analysis with molecular markers. These immunofluorescence experiments allowed us to conclude that localization of asymmetric cell division markers such as Inscuteable, Miranda, Prospero and Numb is cell autonomous. By time-lapse video recording we have observed interesting cellular features of postembryonic neurogenesis such us the polarized genesis of the neuroblast progeny, the extremely short ganglion mother cell (GMC) cell cycle, and the last division of a neuroblast lineage. The combination of this cell culture system and genetic tools of Drosophila will provide a powerful experi...Continue Reading

References

Jan 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K H Jones, J A Senft
Feb 1, 1996·Current Opinion in Neurobiology·C Q Doe, J B Skeath
Jul 1, 1996·Journal of Neurobiology·S E Perez, H Steller
Oct 29, 1997·Current Topics in Developmental Biology·F FeiguinC González
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·J C ClemensJ E Dixon
Jun 9, 2000·Annual Review of Neuroscience·B LuY N Jan
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·J A Knoblich
Nov 8, 2001·Experimental Cell Research·M Schaefer, J A Knoblich
Nov 21, 2001·Nature Reviews. Neuroscience·Y N Jan, L Y Jan
Oct 22, 2003·Cell Motility and the Cytoskeleton·Shawna L Fleming, Conly L Rieder
Apr 28, 2004·Developmental Biology·Barbara Küppers-MuntherAndreas Prokop
Dec 17, 2004·Biology of the Cell·Hongyan Wang, William Chia
Jan 5, 2005·Nature Reviews. Genetics·Ethan Bier
Feb 1, 1990·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Alois Hofbauer, José A Campos-Ortega

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Citations

Apr 20, 2013·Nature Protocols·Boris EggerSimon G Sprecher
May 11, 2013·Nature Protocols·Heike HarzerJuergen A Knoblich
Nov 26, 2008·Oncogene·J Januschke, C Gonzalez
Nov 22, 2013·PloS One·Catarina C F HomemJuergen A Knoblich
Jan 30, 2016·Nature Communications·Rouven DingChristian Berger
Mar 1, 2012·Wiley Interdisciplinary Reviews. Developmental Biology·Phing Chian ChaiYu Cai
Dec 19, 2014·PLoS Genetics·Victoria K SchulmanMary K Baylies
Mar 1, 2018·Genetics·Florian HeigwerMichael Boutros
Nov 8, 2012·Development·Catarina C F Homem, Juergen A Knoblich

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