A probable Na+(K+)/H+ exchanger on the chloroplast envelope functions in pH homeostasis and chloroplast development in Arabidopsis thaliana

Proceedings of the National Academy of Sciences of the United States of America
Chun-Peng SongJian-Kang Zhu

Abstract

Electroneutral monovalent cation/proton antiport across the chloroplast envelope has been shown previously to have an important regulatory effect on stromal pH and thereby on photosynthetic carbon reduction. Here we report that an Arabidopsis nuclear gene, AtCHX23, encodes a putative Na(+)(K(+))/H(+) exchanger and functions in the adjustment of pH in the cytosol and possibly in maintaining a high pH level in the chloroplast stroma. The AtCHX23 protein is localized in the chloroplast envelope. Plastids from chx23 mutants had straight thylakoids but lacked grana lamellae. chx23 mutant leaves were pale yellow and had a much reduced chlorophyll content. The chlorophyll content of chx23 was increased by growing in medium at low (4.0) pH and decreased by growing at high (7.0) pH. The cytosolic pH in the leaves of the mutant was significantly higher than that in the wild type. chx23 mutants displayed a high sensitivity to NaCl. Together, these data indicate that CHX23 is a probable chloroplast Na(+)(K(+))/H(+) exchanger important for pH homeostasis and chloroplast development and function.

References

Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·A T Jagendorf, E Uribe
Apr 18, 1998·The Journal of Biological Chemistry·M NumataJ Orlowski
Mar 31, 2000·Trends in Plant Science·T Cavalier-Smith
Apr 5, 2000·Nature Biotechnology·C M McCallumS Henikoff
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·C F Chuang, E M Meyerowitz
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·H ShiJ K Zhu
Oct 18, 2000·Nature·S Fukada-TanakaS Iida
Mar 15, 2001·Biochimica Et Biophysica Acta·E PadanN Dover
May 31, 2001·Plant & Cell Physiology·T YamaguchiS Iida
Aug 14, 2001·Plant Physiology·P MäserM L Guerinot
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·R A GaxiolaG R Fink
May 23, 2002·Biochemistry
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Quan-Sheng QiuJian-Kang Zhu
Dec 1, 1996·The Plant Cell·S. J. Swanson, R. L. Jones

❮ Previous
Next ❯

Citations

Oct 26, 2005·The Journal of Membrane Biology·I I PottosinS Shabala
Aug 16, 2006·The Journal of Membrane Biology·M A BissonV A Shepherd
Nov 7, 2006·Journal of Molecular Evolution·T ShigakiK D Hirschi
Oct 22, 2010·Planta·Atsunori FukudaYoshiyuki Tanaka
Mar 4, 2011·Plant Molecular Biology·Xiang-Qiang KongHui Zhang
Oct 7, 2011·Journal of Experimental Botany·A R EvansH J Newbury
Jan 31, 2009·Plant & Cell Physiology·Tantravahi SrinivasanPulugurtha Bharadwaja Kirti
Aug 11, 2011·Plant & Cell Physiology·Artur CondeHernâni Gerós
Mar 6, 2007·Plant Physiology·Senthilkumar PadmanabanHeven Sze
Aug 5, 2008·Plant Physiology·Jian ZhaoKendal D Hirschi
May 3, 2005·Annual Review of Plant Biology·Andreas P M WeberUlf-Ingo Flügge
Jul 10, 2013·Cellular and Molecular Life Sciences : CMLS·Bernard E PfeilCornelia Spetea
May 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Hans-Henning KunzJulian I Schroeder
Sep 1, 2012·Annual Review of Genetics·Norbert RollandDaphné Seigneurin-Berny
Jan 22, 2013·Annual Review of Plant Biology·Yi Wang, Wei-Hua Wu
Dec 27, 2011·Plant Science : an International Journal of Experimental Plant Biology·Yoonkang HurHye Ryun Woo
Jul 23, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Juntao Liu, Yan Guo
Sep 29, 2009·Trends in Plant Science·Tomoaki HorieJulian I Schroeder
Feb 17, 2009·Journal of Plant Physiology·Mark W SzczerbaHerbert J Kronzucker
Aug 17, 2005·The New Phytologist·Rana Munns
Aug 21, 2008·Journal of Integrative Plant Biology·Yi-Fang ChenWei-Hua Wu
Mar 19, 2014·Plant, Cell & Environment·Vladimir SukhovVladimir Vodeneev
Apr 20, 2010·Plant Biology·S Chen, A Polle
Dec 3, 2014·Cell Calcium·Giovanni FinazziDaphné Seigneurin-Berny
Apr 5, 2015·Journal of Plant Physiology·Edgar Pinto, Isabel M P L V O Ferreira
May 9, 2014·Journal of Plant Physiology·Izhar Ahmad, Frans J M Maathuis
May 8, 2016·Photosynthesis Research·Vladimir Sukhov
Jun 1, 2016·Journal of Experimental Botany·Thibaut BontpartNancy Terrier
May 10, 2017·International Journal of Molecular Sciences·Jinwei SuoShaojun Dai
Mar 4, 2006·Journal of Experimental Botany·José M PardoFrancisco J Quintero
Oct 2, 2009·Plant Signaling & Behavior·M Pilar Rodríguez-RosalesKees Venema

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.