A probe of the active site acidity of carboxypeptidase A
Biochemistry
May 20, 1986
William L Mock, J T Tsay
PMID: 3718930
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Abstract
The substrate analogue 2-(1-carboxy-2-phenylethyl)-4-phenylazophenol is a potent competitive inhibitor of carboxypeptidase A. Upon ligation to the active site, the azophenol moiety undergoes a shift of pKa from a value of 8.76 to a value of 4.9; this provides an index of the Lewis acidi...read more
Mentioned in this Paper
Azo Compounds
Carboxypeptidase A5
Hydrogen-Ion Concentration
In Vivo NMR Spectroscopy
Carboxypeptidase
2-(1-carboxy-2-phenylethyl)-4-phenylazophenol, (L)-isomer
Paper Details
References
- References19
- Citations4
The pH dependence of peptide hydrolysis by nitrocarboxypeptidase A
Archives of Biochemistry and BiophysicsSeptember 1, 1980William L Mock, J T Chen
The mechanism of the aminolysis of acetate esters
Journal of the American Chemical SocietyOctober 30, 1974Arnold C Satterthwait, William P Jencks
Site-directed mutagenesis shows that tyrosine 248 of carboxypeptidase A does not play a crucial role in catalysis
NatureJanuary 1, 1985Stephen J. GardellWilliam J RutterExtreme state of ionization of benzylsuccinate bound by carboxypeptidase A
BiochemistrySeptember 28, 1982Alan Richard PalmerRichard V Wolfenden
Effects of pH on the structure and function of carboxypeptidase A: crystallographic studies
Proceedings of the National Academy of Sciences of the United States of AmericaDecember 1, 1984Gil ShohamWilliam N. LipscombSpectral properties of cobalt carboxypeptidase. The effects of substrates and inhibitors
BiochemistryNovember 1, 1971S A Latt, Bert L ValleeEffects of metal ion substitution on carboxypeptidase A catalyzed hydrolysis of O-trans-cinnamoyl-L-beta-phenyllactate
BiochemistryJuly 19, 1983Stephanie A King, Thomas H. Fife
Hydrolysis of a thiopeptide by cadmium carboxypeptidase A
Biochemical and Biophysical Research CommunicationsSeptember 16, 1981William L MockJ W TsangKinetics of carboxypeptidase A. The pH dependence of tripeptide hydrolysis catalyzed by zinc, cobalt, and manganese enzymes
BiochemistryOctober 27, 1970Douglas S Auld, Bert L ValleeEnzymatically inactive, exchange-inert Co(III)-carboxypeptidase A: role of inner sphere coordination in peptide and ester catalysis
BiochemistryAugust 8, 1978Harold E Van Wart, Bert L Vallee
12
- References19
- Citations4
The ketone cinnamoyl-(1-13C-Phe)-CGly-Pro-Pro is a tetrahedral transition state analog inhibitor of C. histolyticum collagenase
Biochemical and Biophysical Research CommunicationsMarch 15, 1989D GrobelnyR E Galardy19F nuclear magnetic resonance spectroscopy study of the complex of 2-benzyl-4-oxo-5,5,5-trifluoropentanoic acid and carboxypeptidase A
Biochemical and Biophysical Research CommunicationsJune 16, 1988C TeaterR E GalardySynergistic inhibition of carboxypeptidase A by zinc ion and imidazole
Biochemical and Biophysical Research CommunicationsApril 2, 1999William L Mock, Li Min Wang
Kinetic characterization of the neutral protease vimelysin from Vibrio sp. T1800
European Journal of BiochemistryOctober 15, 1996S KunugiKohei Oda
1
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A probe of the active site acidity of carboxypeptidase A
Abstract
The substrate analogue 2-(1-carboxy-2-phenylethyl)-4-phenylazophenol is a potent competitive inhibitor of carboxypeptidase A. Upon ligation to the active site, the azophenol moiety undergoes a shift of pKa from a value of 8.76 to a value of 4.9; this provides an index of the Lewis acidi...read more
Mentioned in this Paper
Azo Compounds
Carboxypeptidase A5
Hydrogen-Ion Concentration
In Vivo NMR Spectroscopy
Carboxypeptidase
Related Papers
Paper Details
References
- References19
- Citations4
The pH dependence of peptide hydrolysis by nitrocarboxypeptidase A
Archives of Biochemistry and BiophysicsSeptember 1, 1980William L Mock, J T ChenThe mechanism of the aminolysis of acetate esters
Journal of the American Chemical SocietyOctober 30, 1974Arnold C Satterthwait, William P JencksSite-directed mutagenesis shows that tyrosine 248 of carboxypeptidase A does not play a crucial role in catalysis
NatureJanuary 1, 1985Stephen J. GardellWilliam J RutterExtreme state of ionization of benzylsuccinate bound by carboxypeptidase A
BiochemistrySeptember 28, 1982Alan Richard PalmerRichard V WolfendenEffects of pH on the structure and function of carboxypeptidase A: crystallographic studies
Proceedings of the National Academy of Sciences of the United States of AmericaDecember 1, 1984Gil ShohamWilliam N. LipscombSpectral properties of cobalt carboxypeptidase. The effects of substrates and inhibitors
BiochemistryNovember 1, 1971S A Latt, Bert L ValleeEffects of metal ion substitution on carboxypeptidase A catalyzed hydrolysis of O-trans-cinnamoyl-L-beta-phenyllactate
BiochemistryJuly 19, 1983Stephanie A King, Thomas H. FifeHydrolysis of a thiopeptide by cadmium carboxypeptidase A
Biochemical and Biophysical Research CommunicationsSeptember 16, 1981William L MockJ W TsangKinetics of carboxypeptidase A. The pH dependence of tripeptide hydrolysis catalyzed by zinc, cobalt, and manganese enzymes
BiochemistryOctober 27, 1970Douglas S Auld, Bert L ValleeEnzymatically inactive, exchange-inert Co(III)-carboxypeptidase A: role of inner sphere coordination in peptide and ester catalysis
BiochemistryAugust 8, 1978Harold E Van Wart, Bert L Vallee
12
- References19
- Citations4
The ketone cinnamoyl-(1-13C-Phe)-CGly-Pro-Pro is a tetrahedral transition state analog inhibitor of C. histolyticum collagenase
Biochemical and Biophysical Research CommunicationsMarch 15, 1989D GrobelnyR E Galardy19F nuclear magnetic resonance spectroscopy study of the complex of 2-benzyl-4-oxo-5,5,5-trifluoropentanoic acid and carboxypeptidase A
Biochemical and Biophysical Research CommunicationsJune 16, 1988C TeaterR E GalardySynergistic inhibition of carboxypeptidase A by zinc ion and imidazole
Biochemical and Biophysical Research CommunicationsApril 2, 1999William L Mock, Li Min WangKinetic characterization of the neutral protease vimelysin from Vibrio sp. T1800
European Journal of BiochemistryOctober 15, 1996S KunugiKohei Oda
1
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