PMID: 3766968Aug 15, 1986Paper

A procedure for the immunoblotting of proteins separated on isoelectric focusing gels

Analytical Biochemistry
C A OteyJ C Bulinski

Abstract

A method has been devised for performing Western blot assays on proteins resolved by isoelectric focusing. Electrophoretic transfer of proteins directly from isoelectric focusing (IEF) tube gels to nitrocellulose sheets allowed their immunoassay without conventional second dimension SDS gel electrophoresis. The same method can also be used for IEF slab gels. For the immunostaining of nonmuscle actin isoforms in extracts of cultured cells, the resolution of this technique was much improved over that of Western blots of two-dimensional gels.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
May 1, 1986·The Journal of Cell Biology·C A OteyJ C Bulinski
Jan 1, 1986·The Journal of Cell Biology·D A KaiserT D Pollard
Jul 1, 1982·Analytical Biochemistry·M P Reinhart, D Malamud
May 1, 1983·Analytical Biochemistry·J M Gershoni, G E Palade
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J C BulinskiS D Hauschka
Jul 1, 1983·The Journal of Cell Biology·S W L'Hernault, J L Rosenbaum
Apr 28, 1981·Biochemistry·H J GeorgeJ C Lee
Dec 3, 1981·Nature·I Gozes, K J Sweadner

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Citations

Aug 4, 2001·Biochemical and Biophysical Research Communications·T NakataH Yorifuji
Jul 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Hailong LuKathleen M Trybus
Feb 1, 1992·Microscopy Research and Technique·M CamatiniP Bonfanti
Sep 1, 1988·Archives of Biochemistry and Biophysics·P Cheung, E Reisler
Jun 1, 1987·Journal of Cellular Biochemistry·C A OteyJ C Bulinski

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