Dec 1, 1985

A procedure for the purification of ferritin from human liver by heating a methanol-treated homogenate

Analytical Biochemistry
B E ChamK Ridgway


A simple, rapid technique for purification of ferritin from human liver tissue is described. Methanol, at a final concentration of 40% (v/v) in liver homogenate, precipitates the majority of proteins but does not affect ferritin. Subsequent heating of this homogenate at 75 degrees C for 10 min results in a purified ferritin preparation as judged by immunoelectrophoresis and polyacrylamide gel electrophoresis. The resultant purified ferritin contained the same amount of iron as the original endogenous ferritin. There were no significant differences (paired t tests) in the amount of protein in the purified ferritin preparation when measured by rocket immunoelectrophoresis and by the Lowry procedure, suggesting that the antigenecity of ferritin was unaffected by the methanol and heat treatment. Both endogenous liver ferritin and radiolabeled human liver ferritin added to liver homogenates were recovered after methanol and heat treatment with similar yields (77 +/- 7% and 70 +/- 2%, respectively) when compared with the standard treatment of heating a homogenate at 75 degrees C. The overall ferritin yield with this rapid procedure was 40%.

  • References2
  • Citations12


  • References2
  • Citations12


Mentioned in this Paper

Electrophoresis, Agar Gel
Sodium Methoxide
Hot Temperature
Rapid Technique
Liver Parenchyma
High Pressure Liquid Chromatography Procedure
Purification Aspects
Iodine Radioisotopes

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