A Procedure to Design One-Pot Multi-enzyme System for Industrial CDP-Choline Production.

Applied Biochemistry and Biotechnology
Cheng ZhengHanjie Ying

Abstract

Fermentation and chemical methods for industrial cytidine diphosphate choline (CDP-choline) catalytic production both suffer from several disadvantages such as relatively low efficiency and productivity. To overcome these problems, we applied the concept of synthetic biology to develop a new one-pot multi-enzyme system to produce CDP-choline from orotic acid. Enzymes from different sources were selected and optimized as building blocks of the system, and parameters such as oxygen supply were also optimized. This system shows a titer of 37.6 ± 1.1 mM and a reaction rate of 1.6 mM L-1 h-1, both increase 66 % from traditional processes. It also has an efficiency of energy of 25.4%, improves 2-folds. This new one-pot CDP-choline-producing system has a potential for industrial use, and the procedure to design one-pot multi-enzyme system can be applied to build other one-pot system producing energy-rich compounds.

References

Oct 1, 1996·Current Opinion in Biotechnology·C Kurland, J Gallant
Mar 6, 1998·Trends in Biotechnology·G Hannig, S C Makrides
Dec 21, 2004·Journal of Biotechnology·Hans Peter Sørensen, Kim Kusk Mortensen
Oct 19, 2007·Accounts of Chemical Research·Stefaan M A De WildemanOliver May
Dec 20, 2007·Molecular Systems Biology·Luis Serrano
Jan 15, 2010·Current Opinion in Chemical Biology·Gjalt W HuismanAnke Krebber
May 12, 2012·Nature·U T BornscheuerK Robins
Oct 16, 2016·Applied Microbiology and Biotechnology·Yingmiao LiuHanjie Ying

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