A progesterone biosensor derived from microbial screening.

Nature Communications
Chloé GrazonJames E Galagan

Abstract

Bacteria are an enormous and largely untapped reservoir of biosensing proteins. We describe an approach to identify and isolate bacterial allosteric transcription factors (aTFs) that recognize a target analyte and to develop these TFs into biosensor devices. Our approach utilizes a combination of genomic screens and functional assays to identify and isolate biosensing TFs, and a quantum-dot Förster Resonance Energy Transfer (FRET) strategy for transducing analyte recognition into real-time quantitative measurements. We use this approach to identify a progesterone-sensing bacterial aTF and to develop this TF into an optical sensor for progesterone. The sensor detects progesterone in artificial urine with sufficient sensitivity and specificity for clinical use, while being compatible with an inexpensive and portable electronic reader for point-of-care applications. Our results provide proof-of-concept for a paradigm of microbially-derived biosensors adaptable to inexpensive, real-time sensor devices.

References

Nov 6, 2002·Journal of Molecular Biology·Nitzan RosenfeldUri Alon
Mar 26, 2004·Nature Reviews. Microbiology·Martin Keller, Karsten Zengler
Nov 5, 2004·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·N KashtanU Alon
Jun 14, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yoshiaki MinezakiKen Nishikawa
May 19, 2007·Nature Reviews. Genetics·Uri Alon
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Sep 19, 2009·Molecular BioSystems·Ernesto Pérez-RuedaAgustino Martínez-Antonio
Apr 27, 2010·Chemical Society Reviews·Niina J RonkainenWilliam R Heineman
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Nov 6, 2012·Trends in Biotechnology·Anthony Turner
Nov 17, 2012·BMC Genomics·Philippe OrtetMohamed Barakat
Mar 23, 2013·Chemosphere·Chang-Ping YuKung-Hui Chu
Jul 9, 2013·Steroids·R EcochardR Fehring
May 9, 2014·Journal of the American Board of Family Medicine : JABFM·Rene LeivaElizabeth Tanguay
May 24, 2014·Trends in Biotechnology·Amay J Bandodkar, Joseph Wang
Nov 25, 2014·Cell·Keith PardeeJames J Collins
Jan 13, 2015·Genome Announcements·Victoriya Y ShtratnikovaMarina V Donova
Mar 24, 2015·Journal of the American Chemical Society·Wentao WangHedi Mattoussi
Jul 21, 2015·Frontiers in Microbiology·Raul Fernandez-LópezGabriel Moncalián
Sep 17, 2015·Analytical Chemistry·Carly A HolsteinPaul D Sampson
Nov 1, 2015·Methods in Molecular Biology·Sreya Ghosh, Chon-Kit Kenneth Chan
Nov 2, 2015·Applied Microbiology and Biotechnology·Regina Mahr, Julia Frunzke
Dec 23, 2015·Nature Methods·Noah D TaylorSrivatsan Raman
Oct 19, 2016·Current Opinion in Microbiology·Vincent LibisJean-Loup Faulon
Nov 12, 2016·Journal of Industrial Microbiology & Biotechnology·Brecht De PaepeMarjan De Mey
Jul 21, 2017·Nature Protocols·Anna BartlettJoseph R Ecker
Oct 25, 2017·Nanoscale·Margaret ChernAllison M Dennis
Dec 12, 2018·Methods and Applications in Fluorescence·Margaret ChernAllison M Dennis

❮ Previous
Next ❯

Citations

Jul 22, 2020·Advanced Healthcare Materials·Mingfu ChenMark W Grinstaff
Aug 17, 2020·Nucleic Acids Research·Roman S Iwasaki, Robert T Batey
Jan 26, 2021·Current Opinion in Biotechnology·Melissa M MitchlerGavin J Williams
Mar 20, 2021·Emerging Topics in Life Sciences·Mahalakshmi Harish, Prasanna Venkatraman
Apr 11, 2021·Applied and Environmental Microbiology·Shumin XuYu Deng
Jun 19, 2021·Chemical Society Reviews·Dong YiUwe T Bornscheuer
Sep 8, 2020·ACS Applied Materials & Interfaces·Mingfu ChenMark W Grinstaff

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE141603
CP009896.1

Methods Mentioned

BETA
biosensor
biosensors
ChIP-Seq
biolayer interferometry
BioLayer
biosensing
FRET
Assay
RNA-Seq
PCR

Software Mentioned

deseq
matlab script
Cutadapt
Cufflinks
LabView
Galagan Lab

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.