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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity

Science

Jun 30, 2012

Martin JinekEmmanuelle Charpentier

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. We show here that in a subs...read more

Mentioned in this Paper

Study
RNA Conformation
Deoxyribonuclease I
RuvC protein, E coli
Virus
Cleaved
CRISPR-Associated Protein Complex Proteins
Endonuclease
Bacteriophages
Chimera Organism
89
129
14
9358
2
14
1
Paper Details
References
  • References43
  • Citations2467
12345
  • References43
  • Citations2467
12345...

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Proceedings of the National Academy of Sciences of the United States of America

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity

Science

Jun 30, 2012

Martin JinekEmmanuelle Charpentier

PMID: 22745249

DOI: 10.1126/science.1225829

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. We show here that in a subs...read more

Mentioned in this Paper

Study
RNA Conformation
Deoxyribonuclease I
RuvC protein, E coli
Virus
Cleaved
CRISPR-Associated Protein Complex Proteins
Endonuclease
Bacteriophages
Chimera Organism
89
129
14
9358
2
14
1

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CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

Proceedings of the National Academy of Sciences of the United States of AmericaSeptember 6, 2012
Giedrius GasiunasVirginijus Siksnys
Paper Details
References
  • References43
  • Citations2467
12345
  • References43
  • Citations2467
12345...

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