A proline-rich protein binds to the localization element of Xenopus Vg1 mRNA and to ligands involved in actin polymerization

The EMBO Journal
W M ZhaoP W Huber

Abstract

A 340 nucleotide element within the 3' untranslated region of Vg1 mRNA determines its localization to the vegetal cortex of Xenopus oocytes. To identify protein factors that bind to this region, we screened a cDNA expression library with an RNA probe containing this sequence. Five independent isolates encoded a protein (designated Prrp for proline-rich RNA binding protein) having two RNP domains followed by multiple polyproline segments. Prrp and Vg1 mRNAs are co-localized to the vegetal cortex of stage IV oocytes, substantiating an interaction between the two in vivo. Prrp also associates with VegT mRNA, which like Vg1 mRNA uses the late localization pathway, but not with Xcat-2 or Xwnt-11 mRNAs, which use the early pathway. The proline-rich domain of Prrp interacts with profilin, a protein that promotes actin polymerization. Prrp can also associate with the EVH1 domain of Mena, another microfilament-associated protein. Since the anchoring of Vg1 mRNA to the vegetal cortex is actin dependent, one function of Prrp may be to facilitate local actin polymerization, representing a novel function for an RNA binding protein.

References

Aug 25, 1992·Nucleic Acids Research·K B Hall, W T Stump
Jul 12, 1991·Cell·D St JohnstonC Nüsslein-Volhard
Sep 13, 1991·Science·C L Sundell, R H Singer
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C T ChienS Fields
Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·L Breeden, K Nasmyth
Sep 25, 1995·Nucleic Acids Research·I N MaruyamaH I Maruyama
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·R J RicklesM J Zoller
Aug 13, 1993·Cell·G H Thomsen, D A Melton
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·A B SparksB K Kay
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·K L Mowry
Feb 1, 1997·Current Opinion in Cell Biology·G Bassell, R H Singer
Jul 1, 1997·Current Biology : CB·J A Frazier, C M Field
Dec 31, 1997·Biochimica Et Biophysica Acta·K SchlüterM Rothkegel
May 20, 1998·Current Biology : CB·J O DeshlerB J Schnapp
Aug 8, 1998·Trends in Cell Biology·S Wasserman
Sep 17, 1998·The Journal of Biological Chemistry·L Blanchoin, T D Pollard
Oct 6, 1998·Annual Review of Biochemistry·A BashirullahH D Lipshitz
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·X C Fan, J A Steitz
Mar 24, 1999·The Journal of Cell Biology·V LaurentM F Carlier
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S M Ahern-DjamaliF M Hoffmann
Jun 9, 1999·Current Biology : CB·D L BeachK Bloom

❮ Previous
Next ❯

Citations

Oct 29, 2002·Cellular Signalling·Mousumi MajumdarWilliam B Stallcup
Jan 17, 2002·Current Opinion in Cell Biology·Patricia J Renfranz, Mary C Beckerle
Apr 27, 2005·Nature Reviews. Molecular Cell Biology·Daniel St Johnston
Dec 17, 2004·Biology of the Cell·Robert S Cohen
Dec 17, 2004·Biology of the Cell·Mary Lou KingKimberly L Mowry
Dec 17, 2004·Biology of the Cell·Joel K Yisraeli
May 22, 2009·The Journal of Biological Chemistry·Patrick K ArthurTomas Pieler
Nov 5, 2011·Development, Growth & Differentiation·Gaku Kumano
Nov 28, 2007·Molecular and Cellular Biology·Raymond A LewisKimberly L Mowry
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Stefan KindlerHenri Tiedge
Jul 27, 2007·RNA·Froma ObermanJoel K Yisraeli
Feb 20, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Matthew BrookNicola K Gray
Apr 21, 2004·The Journal of Cell Biology·Tracy L KressKimberly L Mowry
Mar 22, 2016·Journal of Developmental Biology·Tristan AgueroMary Lou King
Mar 28, 2008·Critical Reviews in Biochemistry and Molecular Biology·Brian M Farley, Sean P Ryder
Apr 5, 2005·Critical Reviews in Biochemistry and Molecular Biology·Lucy J Colegrove-OteroNancy Standart
May 14, 2004·International Journal of Andrology·Pauline H Yen
Jan 11, 2002·Developmental Biology·J HeasmanD S Kessler
Oct 8, 2005·Fertility and Sterility·Hsien-An PanPao-Lin Kuo
Jul 15, 2015·Developmental Biology·Diana BauermeisterTomas Pieler
Aug 16, 2003·Differentiation; Research in Biological Diversity·Luetta AllenLaurence D Etkin
Jul 5, 2007·Differentiation; Research in Biological Diversity·Kinneret Rand, Joel K Yisraeli
Mar 23, 2002·Cell·Malgorzata KlocLaurence D Etkin
Jun 22, 2005·Current Opinion in Genetics & Development·Svetlana Minakhina, Ruth Steward
Nov 5, 2014·The Journal of Biological Chemistry·Mariam Q MalikPaul W Huber
Feb 12, 2019·Frontiers in Genetics·Vinay K Mayya, Thomas F Duchaine
Sep 13, 2005·Journal of Cell Science·Yaron Shav-Tal, Robert H Singer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.