A promising cancer gene therapy agent based on the matrix protein of vesicular stomatitis virus

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Jumei ZhaoYuquan Wei

Abstract

The matrix (M) protein of vesicular stomatitis virus (VSV) plays a key role in inducing cell apoptosis during infection. To investigate whether M protein-mediated apoptosis could be used in cancer therapy, its cDNA was amplified and cloned into eukaryotic expression vector pcDNA3.1(+). The recombinant plasmid or the control empty plasmid pcDNA3.1(+) was mixed with cationic liposome and introduced into various tumor cell lines in vitro, including lung cancer cell LLC, A549, colon cancer cell CT26 and fibrosarcoma cell MethA. Our data showed that the M protein induced remarkable apoptosis of cancer cells in vitro compared with controls. Fifty micrograms of plasmid in a complex with 250 microg cationic liposome was injected intratumorally into mice bearing LLC or MethA tumor model every 3 days for 6 times. It was found that the tumors treated with M protein plasmid grew much more slowly, and the survival of the mice was significantly prolonged compared with the mice treated with the control plasmid. In MethA fibrosarcoma, the tumors treated with M protein plasmid were even completely regressed, and the mice acquired longtime protection against the same tumor cell in rechallenge experiments. Both apoptotic cells and CD8(+) T cells ...Continue Reading

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Citations

Jul 7, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Raúl OrtizAntonia Aranega
Sep 23, 2009·Cancer Chemotherapy and Pharmacology·Jose PradosAntonia Aránega
Jul 10, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Raúl OrtizAntonia Aranega
Jul 5, 2011·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ana R RamaA Aranega
Sep 26, 2017·ACS Applied Materials & Interfaces·Jianlin LongMaling Gou

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