A promoter ISRE and dual 5' YY1 motifs control IFN-gamma induction of the IRG-47 G-protein gene

Gene
M GillyRandolph Wall

Abstract

The IRG-47 gene is the prototype for a new family of genes encoding guanine nucleotide binding proteins (G-proteins) which are selectively induced in different cell lineages in response to activation signals. The IRG-47 gene is rapidly and transiently induced by interferon-gamma (IFN-gamma) in cells of the B lymphocyte lineage and in stromal cells and fibroblasts. Here we report features controlling the uninduced and IFN-gamma-induced expression of the IRG-47 gene. The minimal IFN-gamma-inducible IRG-47 gene promoter is 96 bp long and contains a TATA box and an ISRE motif with an internal IRF-1/IRF-2 motif. Mutation of the ISRE motif abolishes IFN-gamma induction by the minimal promoter. Constitutively expressed IRF-2 and IFN-gamma-induced IRF-1 factors specifically bind to the wild-type, but not the mutated ISRE motif. An upstream region containing two tandemly repeated YY1 motifs represses the expression of the IRG-47 promoter in uninduced cells and determines the magnitude of IRG-47 promoter activity in IFN-gamma-induced cells. The IRG-47 minimal promoter has the same functional features and organization as the IFN-gamma-inducible promoters of the unrelated murine GBP G-protein multigene family.

References

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Citations

Aug 16, 2012·Functional & Integrative Genomics·Marko Premzl
Jan 17, 2002·Molecular Immunology·Lisa PatroneRandolph Wall
Jun 16, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Nanying CheChuanyou Li
Dec 19, 2007·Immunobiology·Avinash R ShenoyJohn D MacMicking
Feb 8, 2005·Current Opinion in Microbiology·John D MacMicking
Oct 19, 2004·Trends in Immunology·John D MacMicking
Mar 15, 2006·Molecular and Cellular Biochemistry·Tomohiro YamaguchiTakashi Osumi
Oct 9, 1999·The Journal of Biological Chemistry·B M Jacobsen, D G Skalnik

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