A promoter that drives gene expression preferentially in male transgenic rats

Transgenic Research
Qiling LiQing Song

Abstract

Gender-preferential gene expression is a widespread phenomenon in humans. It is important to study how gender differences influence the pathogenesis of various diseases and response to specific drugs. The aim of this study is to determine if the mouse albumin enhancer/promoter may serve as the promoter to introduce gender-preferential gene expression in transgenic animals. We created four independent transgenic rat lines in which the human C-reactive protein transgene was under the control of mouse albumin enhancer/promoter. Quantitative real time RT-PCR analysis showed that transgene expression in the liver of male rats was significantly higher than transgene expression in the female rats (P < 0.05).There was a 5.3-fold (male/female) difference in line-519, and a 12.2-fold (male/female) difference in line-488. Enzyme-linked immunosorbent assay showed that the serum of male transgenic rats had a 13- to 679-fold difference at the protein level on transgene production compared with female transgenic rats. The male-to-female difference in gene expression was 10- to 17-fold in the liver of transgenic rats. Orchiectomy dramatically reduced protein production from the transgene in the liver. Testosterone administration into female ra...Continue Reading

References

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Feb 15, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Kim S SugamoriDenis M Grant

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Citations

Jun 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Qiling LiQing Song
Jul 1, 2020·Clinical Science·Fangfang QiuJian-Xing Ma
Nov 15, 2016·Molecular Medicine Reports·Qiling LiQing Song
Jun 18, 2017·Biological Invasions·Tim Harvey-SamuelLuke Alphey

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