A promoter variant of lncRNA GAS5 is functionally associated with the development of osteosarcoma

Journal of Bone Oncology
Leilei XuShoufeng Wang

Abstract

Previous studies showed that genetic variant rs145204276 in the promoter region of GAS5 was associated with the development of human cancer including colorectal cancer and hepatocellular cancer. This study aimed to investigate the role of rs145204276 in the development of osteosarcoma (OS). 132 OS patients and 1270 healthy controls were recruited for the genotyping analysis of rs145204276. Promoter methylation level of GAS5 was determined for all patients. The tumor tissues and the adjacent normal tissue were collected from 42 patients during surgery and the relative expression of GAS5 was then quantified by Real-time PCR. The Chi-square test was used to determine the difference of genotype and allele frequency between the patients and the controls. The gene expression and the percentage of methylation alleles were compared among different genotypes of rs145204276 with One-way ANOVA test. Compared with the controls, patients were found to have significantly lower rate of genotype del/del (7.6% vs. 8.7%, p = 0.024). The frequency of allele del was significantly lower in the patients than in the controls (23.5% vs. 30.1%, p = 0.021). Compared with than patients with genotype ins/ins, those with genotype del/del had remarkably hig...Continue Reading

Citations

Sep 17, 2019·Technology in Cancer Research & Treatment·Leilei XuShoufeng Wang
May 1, 2021·Journal of Personalized Medicine·Ming-Hong HsiehShun-Fa Yang
Apr 12, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghaforui-Fard, Mohammad Taheri
Sep 16, 2021·The Journal of International Medical Research·Shushan ZhaoLanhua Tang

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Methods Mentioned

BETA
PCR
genotyping

Software Mentioned

QCpG Pyrosequencing
SPSS

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