A proposed role for the SARS-CoV-2 nucleocapsid protein in the formation and regulation of biomolecular condensates

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Sean M Cascarina, Eric D Ross

Abstract

To date, the recently discovered SARS-CoV-2 virus has afflicted >6.9 million people worldwide and disrupted the global economy. Development of effective vaccines or treatments for SARS-CoV-2 infection will be aided by a molecular-level understanding of SARS-CoV-2 proteins and their interactions with host cell proteins. The SARS-CoV-2 nucleocapsid (N) protein is highly homologous to the N protein of SARS-CoV, which is essential for viral RNA replication and packaging into new virions. Emerging models indicate that nucleocapsid proteins of other viruses can form biomolecular condensates to spatiotemporally regulate N protein localization and function. Our bioinformatic analyses, in combination with pre-existing experimental evidence, suggest that the SARS-CoV-2 N protein is capable of forming or regulating biomolecular condensates in vivo by interaction with RNA and key host cell proteins. We discuss multiple models, whereby the N protein of SARS-CoV-2 may harness this activity to regulate viral life cycle and host cell response to viral infection.

References

May 6, 2003·Science·Marco A MarraRachel L Roper
Jul 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Takeshi FukuharaMasatoshi Hagiwara
Aug 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Wataru KamitaniShinji Makino
Feb 23, 2008·Trends in Biochemical Sciences·Paul Anderson, Nancy Kedersha
Oct 20, 2009·Nature Structural & Molecular Biology·Wataru KamitaniShinji Makino
Jan 29, 2010·Journal of Virology·Joanna PiotrowskaKurt E Gustin
May 26, 2010·Antimicrobial Agents and Chemotherapy·Yuko KarakamaMamoru Watanabe
Dec 15, 2010·Journal of Virology·M Simpson-HolleyJ H Connor
Mar 26, 2014·Molecular and Cellular Biology·Daniel K RozelleJohn H Connor
Jul 11, 2014·PLoS Pathogens·Denys A KhaperskyyCraig McCormick
Aug 12, 2014·Viruses·Ruth McBrideBurtram C Fielding
Aug 20, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chong-Shan ShiJohn H Kehrl
Feb 6, 2015·Journal of Virology·Florine E M ScholteMartijn J van Hemert
Sep 16, 2016·EMBO Reports·Karolina Pakos-ZebruckaAdrienne M Gorman
Feb 17, 2017·Proceedings of the National Academy of Sciences of the United States of America·Kimberly L WeirichMargaret L Gardel
Jun 9, 2017·Journal of Virology·Shangmei HouTom C Hobman
Jul 7, 2017·Nature Communications·Jovan NikolicDanielle Blondel
Sep 7, 2017·Cell Reports·Amayra Hernández-VegaAnthony A Hyman

❮ Previous
Next ❯

Citations

Aug 18, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thoru Pederson
Oct 18, 2020·Nucleic Acids Research·Andrea VandelliGian Gaetano Tartaglia
Oct 24, 2020·Journal of Medical Virology·M Shaminur RahmanM Anwar Hossain
Nov 26, 2020·Genetics and Molecular Biology·Fernando Moreira SimabucoArmando Morais Ventura
Nov 29, 2020·Nature Communications·Adriana SavastanoMarkus Zweckstetter
Nov 18, 2020·The EMBO Journal·Theodora Myrto PerdikariNicolas L Fawzi
Dec 15, 2020·Wellcome Open Research·Stefan BressonDavid Tollervey
Dec 3, 2020·Frontiers in Cellular and Infection Microbiology·Rinki MinakshiJihoe Kim
Dec 29, 2020·Biochemical Society Transactions·Ilaria Manfredonia, Danny Incarnato
Jan 22, 2021·Biochemical and Biophysical Research Communications·Mei DangJianxing Song
Feb 9, 2021·Journal of Molecular Biology·Kristina V TugaevaNikolai N Sluchanko
Mar 13, 2021·PLoS Pathogens·Xiaohui JuQiang Ding
Mar 7, 2021·Viruses·Temitope Akhigbe EtiborMaria João Amorim
Apr 29, 2021·Acta Pharmacologica Sinica·Peng-Xuan RenFang Bai
Apr 1, 2021·Genome Génome / Conseil National De Recherches Canada·Jayanta Kumar Das, Swarup Roy
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Danae Campos-MeloMichael J Strong
Jun 24, 2021·Journal of Molecular Biology·Jasmina S RedzicElan Zohar Eisenmesser
Jul 9, 2021·Analytical and Bioanalytical Chemistry·Asif ShajahanParastoo Azadi
Aug 3, 2021·Journal of Biomolecular Structure & Dynamics·Vladimir R Chechetkin, Vasily V Lobzin
Mar 25, 2021·Journal of Molecular Cell Biology·Viviana Scoca, Francesca Di Nunzio
Aug 28, 2021·Signal Transduction and Targeted Therapy·Yu-Wen ZhouXue-Lei Ma

❮ Previous
Next ❯

Software Mentioned

PSP
EMBOSS Needle
catGRANULE

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.