A prospective controlled trial of the optimal volume for neonatal blood cultures

The Pediatric Infectious Disease Journal
Nurit YaacobiRuben Bromiker

Abstract

Bacteremia is a frequent complication in neonatal intensive care units. Blood cultures are the standard for the diagnosis. It is a common practice to draw small blood volumes for culture from neonates in order to prevent anemia; however, this might compromise the test sensitivity. We examined whether using 1 mL of blood in a single aerobic bottle would improve the culture yield compared with our current practice of obtaining 2 samples of 0.5 mL of blood (aerobic and anaerobic bottles). A prospective controlled study was conducted between December 2009 and September 2010 at the neonatal intensive care unit of Shaare Zedek Medical Center, Jerusalem, Israel. Study population included newborns from whom blood cultures were obtained. A sample of 2 mL of blood from each patient was divided into a single aerobic bottle (1 mL; study sample) and into 2 aerobic and anaerobic bottles (0.5 mL each; control samples). Culture bottles were weighed before and after blood inoculation and time to positivity (TTP) was recorded. We obtained 706 complete culture sets from 519 patients. Pathogens grew in 72 (10.2%) cultures from 37 patients. Isolation of organisms was significantly higher in the 0.5 mL control group (94.4% vs. 77.7%, P = 0.012). The...Continue Reading

Associated Clinical Trials

Jun 11, 2015·Bromiker, Ruben M.D

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Citations

Nov 23, 2017·Journal of the Pediatric Infectious Diseases Society·Nathan Messbarger, Kari Neemann
Nov 23, 2017·Journal of the Pediatric Infectious Diseases Society·Sagori Mukhopadhyay, Karen M Puopolo
May 11, 2019·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·D HuggardR K Philip
May 8, 2020·The Pediatric Infectious Disease Journal·Michael W KuzniewiczKaren M Puopolo
Aug 23, 2020·Chinese Medical Journal·Ying DongJean-Christophe Mercier
Oct 18, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S GraspeuntnerJ Pagel
Aug 24, 2018·Frontiers in Pediatrics·Alexa DierigUNKNOWN Swiss Pediatric Sepsis Study
Sep 9, 2020·American Journal of Perinatology·Ruben BromikerGil Klinger
Mar 22, 2020·Archives of Disease in Childhood. Fetal and Neonatal Edition·Moni Pankhuri SinghRajeshwar Reddy Angiti
Oct 28, 2019·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Silke HuberDorothea Orth-Höller
Nov 8, 2020·Seminars in Perinatology·David M RubSagori Mukhopadhyay
Apr 8, 2021·Pediatric Research·Emily C WoodfordSagori Mukhopadhyay
Nov 4, 2021·Pediatric Research·Istemi Han Celik Mohan Pammi

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