PMID: 7520091Sep 1, 1994Paper

A protease-sensitive hinge linking the two domains of the hepatitis B virus core protein is exposed on the viral capsid surface

Journal of Virology
M Seifer, D N Standring

Abstract

Core particles of hepatitis B virus are assembled from dimers of a single 185-residue (subtype adw) viral capsid or core protein (p21.5) which possesses two distinct domains: residues 1 to 144 form a minimal capsid assembly domain, and the arginine-rich, carboxyl-terminal residues 150 to 185 form a protamine-like domain that mediates nucleic acid binding. Little is known about the topography of the p21.5 polypeptide within either the p21.5 capsids or dimers. Here, using site-specific proteases and monoclonal antibodies, we have defined the accessibility of p21.5 residues in dimers and capsids assembled from wild-type and mutant hepatitis B virus core proteins in Xenopus oocytes and in vitro. The data reveal the protamine region to be accessible to external reagents in p21.5 dimers but largely cryptic in wild-type capsids. Strikingly, in capsids the only protease target region was a 9-residue peptide covering p21.5 residues Glu-145 to Asp-153, which falls largely between the two core protein domains. By analogy with protease-sensitive interdomain regions in other proteins, we propose that this peptide constitutes a hinge between the assembly and nucleic acid binding domains of p21.5. We further found that deletion or replacement...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Zhou, D N Standring
Jan 1, 1991·Current Topics in Microbiology and Immunology·D Ganem
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D N StandringW J Rutter
Jun 11, 1981·Nature·J C EdmanW J Rutter
Jan 1, 1983·Annual Review of Immunology·D R Davies, H Metzger
Nov 23, 1978·Nature·S C HarrisonG Bricogne

❮ Previous
Next ❯

Citations

Nov 27, 2009·Virology Journal·Ashraf MohamadkhaniGhodratollah Montazeri
Aug 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arik CooperYosef Shaul
Apr 27, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Yan J PangXiao M Peng
Apr 19, 2006·The Journal of Biological Chemistry·Arik Cooper, Yosef Shaul
Apr 1, 1997·Journal of Structural Biology·R Bringas
Jul 8, 1999·Molecular Cell·S A WynneA G Leslie
Apr 20, 2013·Journal of the American Chemical Society·Jessica Z BereszczakAlbert J R Heck
Nov 22, 2011·Biophysical Journal·Dong MengJianzhong Wu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.