A protective protein antigen of Rickettsia rickettsii has tandemly repeated, near-identical sequences.

Infection and Immunity
B E AndersonR L Regnery

Abstract

The nucleotide sequence of a Rickettsia rickettsii gene that encodes a high-molecular-mass surface antigen (190 kilodaltons), which elicits protective immunity, was determined. The 6,747-nucleotide gene coded for a 2,249-amino-acid protein with a calculated molecular weight of 224,321. A 3.8-kilobase PstI fragment proximal to the 5' end of the gene was found to consist of 13 highly related tandem repeats which constituted over 40% of the coding region. The repeated sequences could be divided into either a 225-nucleotide, 75-amino-acid unit (type I) or a 216-nucleotide, 72-amino-acid unit (type II), with extensive homology between the two types of repeating units. The deduced amino acid sequence for these repeat units, overall, was slightly hydrophobic with short hydrophilic domains. The carboxy-terminal (nonrepetitive) portion of the deduced protein sequence was hydrophilic, with potential surface-exposed epitopes. The full-length reading frame was reconstructed in Escherichia coli, and transient expression of the 190-kilodalton antigen was demonstrated; however, the protein appeared to be severely degraded by proteases and was apparently toxic to E. coli. The conservation of this unique repetitive gene structure, coupled with ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1977·Infection and Immunity·R H KenyonC E Pedersen
Jul 1, 1989·Infection and Immunity·D F HoftL V Kirchhoff
Sep 1, 1989·Journal of Bacteriology·B E Anderson, T Tzianabos
Jun 1, 1987·Journal of Bacteriology·B E AndersonW J Bellini
Aug 1, 1987·Journal of Bacteriology·D O WoodD C Krause
Jul 1, 1988·Molecular and Biochemical Parasitology·C F IbañezA C Frasch
Jul 1, 1988·Journal of Bacteriology·U B Sleytr, P Messner
Feb 11, 1988·Nucleic Acids Research·H ZhangC Somerville
Sep 1, 1988·Journal of Bacteriology·J D DubreuilT J Trust
Dec 1, 1987·The Journal of Experimental Medicine·A E Wallis, W R McMaster
Jun 1, 1985·The Journal of Infectious Diseases·R L AnackerL A Thomas
Jun 1, 1974·Journal of Bacteriology·E L PalmerJ F Obijeski
Oct 1, 1974·Applied Microbiology·E L PalmerL Mallavia
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
Sep 1, 1983·Microbiological Reviews·T J SilhavyS D Emr
Jan 1, 1983·Annual Review of Microbiology·U B Sleytr, P Messner
Apr 1, 1984·Southern Medical Journal·D H WalkerG R Tringali
Feb 7, 1980·Nature·D E BüchelB Müller-Hill
May 11, 1982·Nucleic Acids Research·G D StormoL M Gold
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle

❮ Previous
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Citations

May 1, 1991·European Journal of Epidemiology·L P Mallavia
Jan 1, 1990·Annals of the New York Academy of Sciences·R Regnery
Jan 1, 1994·Microbiology and Immunology·T UchiyamaD H Walker
Jul 16, 2003·Annals of the New York Academy of Sciences·P Renesto, D Raoult
Apr 7, 2005·Applied and Environmental Microbiology·Gerald D BaldridgeUlrike G Munderloh
Feb 17, 2006·Annals of the New York Academy of Sciences·Yanmei JiaoLing Qiu
Dec 30, 2016·Journal of Bacteriology·Nicholas F NorieaTed Hackstadt
Jan 13, 2006·Journal of Molecular Evolution·Francis M Jiggins
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Ian R HendersonDlawer Ala'Aldeen
Jun 27, 2006·Vector Borne and Zoonotic Diseases·Andrea S Varela-StokesSusan E Little
Sep 5, 2007·Experimental & Applied Acarology·Richard C PachecoMarcelo B Labruna
Sep 24, 1998·Infection and Immunity·E KozarovA Progulske-Fox
Sep 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ulrike G Munderloh
Jan 28, 1999·Journal of Bacteriology·M CharlesM B Goldberg
Oct 1, 1992·Journal of Clinical Microbiology·E ManorR Regnery
Mar 1, 1991·Infection and Immunity·S K DuttaB L Mattingly-Napier
May 9, 1998·Journal of Clinical Microbiology·S J WellerT J Kurtti
Dec 10, 2003·Journal of Clinical Microbiology·Marina E EremeevaDavid J Silverman

❮ Previous
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