PMID: 4318781Sep 1, 1970

A protein binding assay for adenosine 3':5'-cyclic monophosphate

Proceedings of the National Academy of Sciences of the United States of America
A G Gilman

Abstract

A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10(-9) M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.

References

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Citations

Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·A L SteinerC Parker
Apr 4, 1969·Analytical Biochemistry·N D GoldbergA G O'Toole
May 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·M Kuwano, D Schlessinger
Aug 1, 1964·Biochemistry·J B POSNERE G KREBS
Dec 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·B M BRECKENRIDGE

Related Concepts

Filtration
Chromatography, DEAE-Cellulose
Plasma Protein Binding Capacity
Tritium
Cyclic AMP, (R)-Isomer
Brain Chemistry
Muscle
Phosphotransferases
Bos indicus
Adenine Nucleotides

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