PMID: 8602359Feb 1, 1996Paper

A protein binds the selenocysteine insertion element in the 3'-UTR of mammalian selenoprotein mRNAs

Nucleic Acids Research
N HubertA Krol

Abstract

Several gene products are involved in co-translational insertion of selenocysteine by the tRNA(Sec). In addition, a stem-loop structure in the mRNAs coding for selenoproteins is essential to mediate the selection of the proper selenocysteine UGA codon. Interestingly, in eukaryotic selenoprotein mRNAs, this stem-loop structure, the selenocysteine insertion sequence (SECIS) element, resides in the 3'-untranslated region, far downstream of the UGA codon. In view of unravelling the underlying complex mechanism, we have attempted to detect RNA-binding proteins with specificity for the SECIS element. Using mobility shift assays, we could show that a protein, present in different types of mammalian cell extracts, possesses the capacity of binding the SECIS element of the selenoprotein glutathione peroxidase (GPx) mRNA. We have termed this protein SBP, for Secis Binding Protein. Competition experiments attested that the binding is highly specific and UV cross-linking indicated that the protein has an apparent molecular weight in the range of 60-65 kDa. Finally, some data suggest that the SECIS elements in the mRNAs of GPx and another selenoprotein, type I iodothyronine 5' deiodinase, recognize the same SBP protein. This constitutes the...Continue Reading

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Citations

Apr 13, 2000·Journal of Cellular Biochemistry·R WuP E Newburger
Sep 25, 2001·BioFactors·P R Copeland, D M Driscoll
May 27, 1999·Journal of Biomedical Science·V N Gladyshev, D L Hatfield
Jul 2, 2003·Neurotoxicity Research·B H Juurlink
Apr 25, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Perrine CastetsValérie Allamand
May 26, 2009·Molecules and Cells·Youn Wook ChungIck Young Kim
Apr 15, 2003·The Proceedings of the Nutrition Society·John E Hesketh, Stéphane Villette
Jul 25, 2000·Nucleic Acids Research·D FagegaltierA Krol
Jan 14, 2003·Annual Review of Nutrition·Donna M Driscoll, Paul R Copeland
Nov 15, 2000·Biological Chemistry·J KöhrlL Flohé
Jan 18, 2006·Cellular and Molecular Life Sciences : CMLS·K Caban, P R Copeland
Apr 21, 2007·Science in China. Series C, Life Sciences·Liang JiangHuiBi Xu
Mar 19, 2004·General and Comparative Endocrinology·Margareta SutijaJean M P Joss
Jun 1, 1996·Trends in Biochemical Sciences·S C Low, M J Berry
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Jun 2, 2006·Biochimie·C Allmang, A Krol
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Nov 18, 2005·Insect Biochemistry and Molecular Biology·Raquel Cossío-BayúgarPatricia J Holman
Oct 30, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Wenbo ChenHaiyan Dong

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