PMID: 2118498Aug 1, 1990Paper

A protein-free medium for the growth of hybridomas and other cells of the immune system

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
F J Darfler

Abstract

A protein-free medium, termed ABC, has been developed which essentially eliminates the need for serum proteins. ABC supports the long-term growth of murine hybridomas as well as other transformed cells of the immune system. The requirement of hybridoma growth for transferrin has been met by substituting the soluble organo-iron compound, sodium nitroprusside. Substantial improvement in the growth of hybridomas was afforded by the inclusion of 18 trace elements complexed to disodium ethylene diaminetetraacetate (EDTA). The medium was further improved by the inclusion of components not found in Ham's F12 medium or by raising the concentrations of existing low molecular weight components. Murine hybridomas can be cultured routinely in this protein-free medium in an anchorage-independent manner with doubling times generally under 24 h. Visualized on electrophoretic gels, levels of monoclonal antibody taken from those cultures often exceeded 80% of the total protein. The medium was also able to support the growth of HuT 78 and H9 cells as well as certain other transformed cells of the immune system. In addition, normal human peripheral blood lymphocytes, activated with phytohemagglutinin and cultured with 50 U/ml recombinant interleu...Continue Reading

References

Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W ShiveK S Matthews
Feb 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G K InnesK E Hobbs
Jun 28, 1988·Journal of Immunological Methods·B LeshemE Kedar
Feb 26, 1987·Journal of Immunological Methods·S P ColeB G Campling
May 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J S ZiglerI Gery
Jul 1, 1982·British Journal of Haematology·C E Gauwerky, D W Golde
Jan 28, 1983·Journal of Immunological Methods·W L ClevelandB F Erlanger
Apr 1, 1983·Journal of Cellular Physiology·F J Darfler, P A Insel
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T R BreitmanS J Collins
Jan 1, 1980·Journal of Immunological Methods·T H ChangH Koprowski
Feb 1, 1982·Journal of Cellular Physiology·M C TsaoR G Ham
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H MurakamiT Kano-Sueoka
Mar 1, 1982·International Journal of Pediatric Otorhinolaryngology·J RuokonenH Lehti
Dec 1, 1980·Cell·D Barnes, G Sato
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·R G HAM

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Citations

Apr 5, 1994·Biotechnology and Bioengineering·C E SandstromE T Papoutsakis
Jan 1, 1993·Cytotechnology·Z ChenY Chen
Aug 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·F J Darfler
Sep 1, 1996·In Vitro Cellular & Developmental Biology. Animal·J Keenan, M Clynes
Nov 13, 2008·Cytotechnology·Joanne KeenanMartin Clynes
Jul 14, 1998·In Vitro Cellular & Developmental Biology. Animal·J G BrunoJ L Kiel
Mar 11, 1992·Biologicals : Journal of the International Association of Biological Standardization·J CinatlH W Doerr
Jan 17, 2020·Nature Protocols·Shay VimerMichal Sharon

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