PMID: 2105741Jan 16, 1990Paper

A protein isolated from Brucella abortus is a Cu-Zn superoxide dismutase

Biochemistry
B L BeckJ E Mayfield

Abstract

Brucella abortus contains a protein that elicits an antigenic response in cattle previously exposed to the organism. The amino acid sequence of the recombinant form of this antigenic protein was determined by gas-phase sequencing of the pyridylethylated protein and its peptides obtained by digestion with cyanogen bromide (CNBr), clostripain, and Staphylococcus aureus V8 protease. The Brucella protein demonstrated 53.6% identity with the Cu-Zn superoxide dismutase (SOD) from Photobacterium leiognathi. Residues essential for metal coordination and enzymatic activity and cysteines required for the formation of the intrasubunit disulfide bridge of Cu-Zn SOD were conserved in the Brucella protein. also exhibited SOD activity that was inhibited by cyanide, which is characteristic of a Cu-Zn SOD. Brucella abortus Cu-Zn SOD is the second prokaryotic Cu-Zn SOD to be sequenced, and the fifth found in prokaryotes. The high degree of conservation between Photobacterium and Brucella Cu-Zn SOD supports the hypothesis of a separately evolved prokaryotic and eukaryotic Cu-Zn SOD gene.

References

May 1, 1989·Veterinary Microbiology·L B TabatabaiJ M Patterson
Jan 1, 1988·Annals of the New York Academy of Sciences·G Minotti
Jan 1, 1988·Free Radical Biology & Medicine·P A WardK J Johnson
Apr 2, 1974·Archives of Biochemistry and Biophysics·F J Yost, I Fridovich
Jun 4, 1974·Biochemical and Biophysical Research Communications·K Puget, A M Michelson
Jan 1, 1984·Annual Review of Microbiology·L Beaman, B L Beaman
Dec 7, 1984·Journal of Chromatography·B A BidlingmeyerT L Tarvin
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W J Wilbur, D J Lipman
Oct 16, 1980·Biochemical and Biophysical Research Communications·C L Borders, J T Johansen

❮ Previous
Next ❯

Citations

Jan 1, 1993·Molecular Aspects of Medicine·R H Burdon
Feb 15, 1991·Veterinary Microbiology·N SriranganathanH Misra
Feb 1, 1994·Veterinary Microbiology·T J StabelJ E Mayfield
Jan 1, 1996·Free Radical Biology & Medicine·L T BenovI Fridovich
Jun 1, 1993·Trends in Microbiology·C L BaldwinD M Fernandes
Aug 15, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Martha Cecilia Moreno-LafontLeopoldo Santos-Argumedo
Nov 1, 1996·Veterinary Immunology and Immunopathology·G SplitterJ Covert
May 23, 2002·FEMS Microbiology Letters·Murielle Roux, Jacques Covés
Dec 3, 1999·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·P Dolashka-AngelovaW Voelter
Sep 20, 1996·Journal of Chromatography. B, Biomedical Applications·W P Michalski
Dec 24, 2011·Veterinary Microbiology·Kyung Yuk KoJi-Yeon Kim
Jan 30, 2008·Veterinary Microbiology·Mohamed N SeleemNammalwar Sriranganathan
Apr 15, 1997·Archives of Biochemistry and Biophysics·L BenovI Fridovich
May 6, 2003·Annual Review of Microbiology·R Martin RoopMalcolm E Winkler
Feb 15, 1996·FEMS Microbiology Letters·J CanvinJ S Kroll
May 11, 2018·Current Medicinal Chemistry·Yuming DengQisheng Peng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.