PMID: 9427517Jan 14, 1998Paper

A protein kinase C-activating phorbol ester enhances transcription of the human DBH gene through a cyclic AMP response element in SK-N-BE(2)C cells

Brain Research. Molecular Brain Research
J S KimK T Kim

Abstract

Protein kinase C (PKC) activation after treatment of human neuroblastoma SK-N-BE(2)C cells with phorbol 12-myristate 13-acetate (PMA) was found to enhance transcription of the human dopamine beta-hydroxylase (DBH) in those cells. To identify which cis-acting element is responsive to the PMA treatment during DBH gene expression, we employed transient transfection assays with serially deleted constructs of the human DBH gene's 5' upstream region fused to the chloramphenicol acetyltransferase (CAT) gene. Treatment of transfected cells with PMA resulted in an approximate threefold increase in CAT expression for all deletion constructs ranging from -978 bp to -262 bp, while the enhancement did not occur with a construct shortened to -114 bp. The region between -262 and -114 bp from the initiation site of transcription contains several cis-regulatory elements including a cyclic AMP response element (CRE) and putative AP1 and YY1 sequences. Site-directed mutagenesis of those cis-acting elements were performed to identify which of the elements mediated the PMA-induced transcriptional enhancement. Substitution of bases in the putative AP1 site containing in part a putative YY1 sequence did not effect the PMA inducibility. However, speci...Continue Reading

References

May 1, 1990·Trends in Neurosciences·M R MontminyK K Yamamoto
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L A KobierskiM J Comb
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E RozengurtM Collins
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Jan 17, 1995·Biochemical and Biophysical Research Communications·H D Chae, K T Kim
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Jul 1, 1996·Brain Research. Molecular Brain Research·J S KimK T Kim
Dec 27, 1996·The Journal of Biological Chemistry·E M SmythG A FitzGerald

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