A protein less sensitive to trypsin, guanidinated casein, is a potent stimulator of exocrine pancreas in rats

Proceedings of the Society for Experimental Biology and Medicine
H HaraT Kasai


Previously, we have shown that, in rats that have had bile-pancreatic juice (BPJ) diverted from the proximal small intestine for 7 days, the exocrine pancreatic secretion was enhanced after they were fed a casein, fat-free diet. This demonstrates that the pancreatic secretion is stimulated by dietary protein with a pancreatic protease-independent pathway. To examine the chemical structure of casein responsible for the enhancement of pancreatic secretion, we prepared chemically modified casein in which lysine residues were guanidinated. Secretion of protein, amylase, and chymotrypsin in the chronic BPJ-diverted rat was increased much more after the rats were fed a diet containing guanidinated casein (250 g/kg diet) than after they were fed a diet containing intact casein (250 g/kg diet). In normal rats whose diverted BPJ was returned to the duodenum, the increases in the pancreatic secretion after consuming the guanidinated casein diet were comparable to those after consuming the intact casein diet. In vitro digestibility of guanidinated casein by trypsin and chymotrypsin was much lower than that of intact casein. Also, guanidinated casein inhibited tryptic hydrolysis of benzoyl-L-arginine p-nitroanilide to a lesser extent than ...Continue Reading


Apr 20, 2001·International Journal of Biological Macromolecules·M SitohyT Haertlé
Mar 17, 2000·Proceedings of the Society for Experimental Biology and Medicine·H HaraT Kasai
Nov 16, 2010·Journal of Proteomics·María Jesús García-MurriaManuel M Sánchez del Pino
Nov 17, 2007·The British Journal of Nutrition·Carlos A MontoyaJean-Paul Lallès

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