PMID: 9445030Jan 28, 1998Paper

A protein linkage map of the P2 nonstructural proteins of poliovirus

Journal of Virology
A CuconatiEckard Wimmer

Abstract

The yeast two-hybrid system was used to catalog all detectable interactions among the P2 nonstructural cleavage products of poliovirus type 1 (Mahoney). Evidence has been obtained for specific associations among 2A(pro), 2BC, 2C, and 2B. Specifically, 2A(pro) can interact with itself and 2BC and its cleavage products (2B and 2C) interact in all possible combinations, with the exception of 2C/2C. Detected interactions were confirmed in vitro by a glutathione S-transferase pulldown assay, which allowed us to detect 2C/2C association. transdominant-negative mutants of 2B (K. Johnson and P. J. Sarnow, J. Virol. 65:4341-4349, 1991) were examined and were found to retain interaction with wild-type 2B, perhaps reflecting a need for 2B multimerization in viral RNA replication. The multimerization of 2B was examined further by screening a mutagenized library for 2B variants that have lost the ability to bind wild-type 2B. The screen identified two nonconservative missense mutations within a central hydrophobic region, as well as truncations and frameshifts that implicate the C terminus in homointeraction. Introduction of the missense mutations into the genome of the virus conferred a quasi-infectious phenotype, an observation strongly s...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S J Hambidge, P Sarnow
Dec 13, 1991·Science·A MollaE Wimmer
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M P ShepleyH L Weiner
Dec 1, 1986·Journal of Virology·H D BernsteinD Baltimore
Jan 1, 1987·Advances in Virus Research·N Sonenberg
Mar 1, 1984·Journal of Virology·M A PallanschR R Rueckert
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·L AlexanderE Wimmer
Oct 1, 1995·Molecular and Cellular Biology·J EstojakE A Golemis
Aug 28, 1995·FEBS Letters·A Barco, L Carrasco
Apr 28, 1995·The Journal of Biological Chemistry·P L Rodríguez, L Carrasco
Jan 1, 1993·Annual Review of Genetics·E WimmerX Cao
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L Van AelstM Wigler
Jan 1, 1996·Nature Genetics·P L BartelS Fields
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·R K BrachmannJ D Boeke
May 1, 1996·Genes & Development·L J Ko, C Prives
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·D J SenGuptaM Wickens
Sep 20, 1996·The Journal of Biological Chemistry·R AldabeL Carrasco
Jan 6, 1997·Virology·F J Van kuppeveldJ R Doedens
Apr 1, 1997·Journal of Virology·G Kadaré, A L Haenni

❮ Previous
Next ❯

Citations

Feb 21, 2004·The Journal of Biological Chemistry·Michelangelo CampanellaFrank J M van Kuppeveld
Sep 7, 2007·Journal of Virology·Matthew P Taylor, Karla Kirkegaard
Jun 13, 2009·The Journal of Biological Chemistry·Peter AdamsEllie Ehrenfeld
Mar 22, 2002·The Journal of General Virology·Arjan S de JongFrank J M van Kuppeveld
Sep 16, 2003·FEBS Letters·José L NievaLuis Carrasco
Mar 29, 2003·Journal of Virology·Julie R Harris, Vincent R Racaniello
May 16, 2006·Journal of Virology·Natalya L TeterinaEllie Ehrenfeld
Sep 16, 2003·FEBS Letters·Maria Eugenia Gonzalez, Luis Carrasco
Aug 17, 2002·The Journal of Biological Chemistry·Aitziber AgirreJosé L Nieva
Aug 27, 2009·Molecular Membrane Biology·George PatargiasWolfgang B Fischer
Aug 16, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Christina Schindler, Wolfgang B Fischer
Jul 13, 2012·Journal of Virology·Mónica González-MagaldiFrancisco Sobrino
Oct 28, 2015·PloS One·Mónica González-MagaldiFrancisco Sobrino
Sep 28, 2005·The Journal of General Virology·Roland ZellPeter Wutzler
Feb 22, 2014·The Journal of General Virology·Chunling WangAniko V Paul
Feb 15, 2008·The Journal of General Virology·Encarnación Martínez-SalasNoemi Fernandez
Sep 20, 2018·PLoS Pathogens·Hongxin GuanSheng Cui
Jan 25, 2006·The Journal of General Virology·William J KaiserIan G Goodfellow
Jul 12, 2007·The Journal of General Virology·Jiang YinAniko V Paul
Oct 3, 1999·Virus Research·V I AgolE Wimmer
Aug 28, 2021·Microorganisms·Ines Cordeiro FilipeCaroline Tapparel
May 26, 2007·Virology Journal·Marion S FreistadtKaren E Eberle
Nov 3, 2009·Virus Research·Kumiko IshikawaKoki Taniguchi
May 19, 2010·Biochimica Et Biophysica Acta·Kai WangBing Sun
Oct 19, 2004·Genome Research·Peter UetzJürgen Haas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.