A protein profile study to discriminate CIN lesions from normal cervical epithelium.

Cellular Oncology (Dordrecht)
Kai-Erik UlebergJan P A Baak

Abstract

Cervical intraepithelial neoplasia (CIN), a frequently encountered disease caused by Human Papilloma Virus (HPV) is often diagnosed in formaldehyde-fixed paraffin embedded (FFPE) punch biopsies. Since it is known that this procedure strongly affects the water-soluble proteins contained in the cervical tissue we decided to investigate whether a water-soluble protein-saving biopsy processing method can be used to support the diagnosis of normal and CIN. Cervical punch biopsies from 55 women were incubated for 24 h at 4°C in RPMI1640 medium for protein analysis prior to usual FFPE processing and p16 and Ki67-supported histologic consensus diagnosis was assessed. The biopsy supernatants were subjected to surface-enhanced laser desorption-ionization time of flight mass spectrometry (SELDI-TOF MS) for identifying differentially expressed proteins. Binary logistic regression and classification and regression trees (CART) were used to develop a classification model. The age of the patients ranged from 26 to 40 years (median 29.7). The consensus diagnoses were normal cervical tissue (n = 10) and CIN2-3 (n = 45). The mean protein concentration was 1.00 and 1.09 mg/ml in the normal and CIN2-3 group, respectively. The peak detection and cl...Continue Reading

References

Apr 1, 1993·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·A G Ostör
Aug 19, 1999·The Journal of Pathology·J M WalboomersN Muñoz
May 5, 2000·The Cochrane Database of Systematic Reviews·P L Martin-HirschH Kitchener
Apr 18, 2002·Journal of Clinical Immunology·Philip E CastlePeggy A Crowley-Nowick
Apr 23, 2004·Current Opinion in Biotechnology·Emanuel F Petricoin, Lance A Liotta
Jul 6, 2004·Clinical Biochemistry·Karin D Rodland
Jul 31, 2004·Urologic Oncology·Emanuel F PetricoinLance A Liotta
Nov 16, 2004·Proteomics·Angelika GörgMichael J Dunn
Feb 1, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Haleem J IssaqTimothy D Veenstra
Aug 11, 2005·Molecular & Cellular Proteomics : MCP·Brian L HoodDavid B Krizman
Dec 15, 2005·Methods in Molecular Medicine·Jan P A Baak, Arnold-Jan Kruse
Jun 15, 2006·Briefings in Functional Genomics & Proteomics·Brian L HoodTimothy D Veenstra
Jul 22, 2006·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·A E PollackL Denny
Nov 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Martijn DijkstraRitsert C Jansen
Dec 10, 2008·Journal of Proteomics·Gitika PanickerElizabeth R Unger
Apr 1, 2003·Analytical Chemistry·Haleem J IssaqTimothy D Veenstra
Sep 1, 2009·Proteomics·Maria Filippa AddisSergio Uzzau
Dec 24, 2009·Journal of Biomedicine & Biotechnology·Muriel De BockMarianne Fillet

❮ Previous
Next ❯

Citations

May 1, 2015·Journal of Lipid Research·Rochelle K Nelson, Michael A Frohman
Sep 13, 2014·PloS One·Geert A A Van RaemdonckXaveer W M Van Ostade
Mar 5, 2016·Oncotarget·Sulma I MohammedJoseph M K Irudayaraj
Oct 15, 2013·Expert Review of Proteomics·Fabio Di DomenicoMarzia Perluigi
Jul 11, 2014·Oncology Letters·Norma Angélica Galicia CanalesJulieta Ivone Castro-Romero

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
biopsies
chip

Software Mentioned

MedCalc
SPSS
CART
Ciphergen Seldi

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.